Effects of Acupuncture and Moxibustion on TGF-β1 mRNA. VEGF mRNA Expression and Apoptosis Gene Fas in Bone Tissue of the Ovariectomized Rats

Liu Xianxiang.Shen Lin.Wu Mingxia.et al.Fujian College of TCM.Fuzhou 350003

doi:10.13288/j.11-2166/r.2003.11.020

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Effects of Acupuncture and Moxibustion on TGF-β1 mRNA. VEGF mRNA Expression and Apoptosis Gene Fas in Bone Tissue of the Ovariectomized Rats

更新时间：2021-11-22

- Effects of Acupuncture and Moxibustion on TGF-β1 mRNA. VEGF mRNA Expression and Apoptosis Gene Fas in Bone Tissue of the Ovariectomized Rats
- Journal of Traditional Chinese Medicine Issue 11, Pages: 830-832(2003)
- 作者机构：
  
  1. 福建中医学院
  2. 华中科技大学同济医学院
  3. 福建省第二人民医院
  4. 华中科技大学同济医学院福州市五四路282号,350003
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2003.11.020
  CLC： R245
- Published：2003
- 稿件说明：
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[1]刘献祥,沈霖,吴明霞,吴炳煌,高兰,胡文胜.针灸对去卵巢大鼠骨组织TGF-β_1 mRNA、VEGF mRNA表达及凋亡基因Fas的影响[J].中医杂志,2003(11):830-832+5.

Liu Xianxiang.Shen Lin.Wu Mingxia.et al.Fujian College of TCM.Fuzhou 350003. Effects of Acupuncture and Moxibustion on TGF-β1 mRNA. VEGF mRNA Expression and Apoptosis Gene Fas in Bone Tissue of the Ovariectomized Rats[J]. Journal of Traditional Chinese Medicine2003, (11): 830-832.
[1]刘献祥,沈霖,吴明霞,吴炳煌,高兰,胡文胜.针灸对去卵巢大鼠骨组织TGF-β_1 mRNA、VEGF mRNA表达及凋亡基因Fas的影响[J].中医杂志,2003(11):830-832+5. DOI： 10.13288/j.11-2166/r.2003.11.020.

Liu Xianxiang.Shen Lin.Wu Mingxia.et al.Fujian College of TCM.Fuzhou 350003. Effects of Acupuncture and Moxibustion on TGF-β1 mRNA. VEGF mRNA Expression and Apoptosis Gene Fas in Bone Tissue of the Ovariectomized Rats[J]. Journal of Traditional Chinese Medicine2003, (11): 830-832. DOI： 10.13288/j.11-2166/r.2003.11.020.

摘要

目的:探讨针灸对去卵巢大鼠骨质疏松症的作用机制和环节。方法:骨组织TGF-β

mRNA、VEGF mRNA采用原位杂交

凋亡基因Fas采用免疫组织化学检测。结果:模型组TGF-β

、VEGF光度低于假手术组（P

0.05）

针刺组、艾灸组、雌二醇组TGF-β

、VEGF光度高于模型组（P

0.05）;骨组织FAS蛋白光度

模型组高于假手术组（P

0.01）

针刺组、艾灸组和雌二醇组低于模型组（P

0.05～0.01）;成骨细胞内FAS蛋白阳性表达率模型组高于假手术组（P

0.01）

雌二醇组低于模型组（P

0.01）;破骨细胞内FAS蛋白阳性表达率针刺组、艾灸组和雌二醇组高于假手术组（P

0.05～0.01）。结论:针灸能有效调节破骨细胞内Fas基因的表达;能激活TGF-β

、VEGF活性

使TGF-β

mRNA、VEGF mRNA表达增加。

Abstract

Objective: To explore the mechanism of acupuncture and moxibustion for treatment of osteoporosis in ovariectomized rats. Methods: TGF-β1

mRNA. VEGF mRNA expression of bone tissue and apoptosis gene Fas were detected respectively by in situ hybridization and immunohistochemistry. Results: TGF-β1 and VEGF gray density in the model group were lower than those in the sham operation group （P<0. 05）

and those in the acupuncture group

the moxibustion group and the estradiol group were higher than those in the model control group （P< 0. 05）. Fas protein positive expression rate in osteoblast in the model group was ligher than that in the sham operation group （P<0. 01）. and in the estradiol group was lower than that in the model group （P<0. 01）. Fas protein positive expression rate in the osteoclast in the acupuncture group

moxibustion group and estradiol group were higher than those in the sham operation group （P < 0.05 - 0.01）. Conclusion: Acupuncture and moxibustion can effectively regulate Fas gene expression in osteoblast. stimulate activities of TGF-β1 and VEGF

and increase expression of TGF-β1

mRNA and VEGF mRNA.

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