Objective:To probe into the method to establish model of syndrome of cold limbs and observe the effect of Sini Capsule on the model.Methods:Dosages and time points of posterior pituitary hormones and nitroglycerin in modeling of syndrome of cold limbs were determined according to changes of voltage of J point in electrocardiogram（ECG）

and changes of temperature of limbs and ECG and the effects of Sini capsule in the model were investigated.Results:Injection of posterior pituitary hormones followed by injection of nitroglycerin could establish the model of syndrome of cold limbs;Sini capsule could block the decrease of temperature of limbs in the model and improve the ischemic changes of ECG.Conclusion:This model is very similar to the syndrome of cold limbs;Sini capsule has the function of recuperating depleted Yang and rescuing the mouse from collapse for the model of syndrome of cold limbs.