Objective:To investigate effects of strengthening the spleen and tonifying the kidney and their combination before and after exercise on relative indexes of metabolism of glucose and amino acids.Methods:Strengthening the spleen alone

tonifying the kidney alone or their combination before and after exercise was given to the rat receiving 7-week gradually increasing load exercise on a running platform

and after quantitative load

contents of serum lactic acid

urea nitrogen

glucose

hepatic glycogen

muscle glycogen

hemoglobin

and malondialdehyde（MDA）in renal tissue and activity of superoxide dismulase（SOD）in the renal tissue were determined.Results:Strengthening the spleen before exercise increased hepatic and muscle glycogen contents

and tonifying the kidney before exercise increased muscle glycogen content

and strengthening the spleen combined with tonifying the kidney before and after exercise increased hemoglobin level

strengthening the spleen followed by tonifying the kidney before exercise decreased serum lactic acid and urea nitrogen levels

increased muscle glycogen content and SOD activity/MDA content ratio in the kidney.Conclusion:Strengthening the spleen and tonifying the kidney play a certain role in reducing motion-related fatigue

while strengthening the spleen followed by tonifying the kidney before exercise has the best results.