Effect of Danshao Huaxian Capsule on Proliferation of Activated Hepatic Stellate Cells in vivo and in vitro

Yao Yumei Geng Xiaoxia Wu Yayun

doi:10.13288/j.11-2166/r.2005.10.030

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Effect of Danshao Huaxian Capsule on Proliferation of Activated Hepatic Stellate Cells in vivo and in vitro

更新时间：2021-11-20

- Effect of Danshao Huaxian Capsule on Proliferation of Activated Hepatic Stellate Cells in vivo and in vitro
- Journal of Traditional Chinese Medicine Issue 10, Pages: 780-782(2005)
- 作者机构：
  
  1. 贵阳医学院附属医院感染科
  2. 贵阳医学院附属医院感染科贵州省贵阳市贵医街28号,550004
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2005.10.030
  CLC： R285.5
- Published：2005
- 稿件说明：
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[1]姚玉梅,耿晓霞,吴亚云,程明亮.丹芍化纤胶囊对体内外活化肝星状细胞增殖的影响[J].中医杂志,2005(10):780-782+790.

Yao Yumei Geng Xiaoxia Wu Yayun. Effect of Danshao Huaxian Capsule on Proliferation of Activated Hepatic Stellate Cells in vivo and in vitro[J]. Journal of Traditional Chinese Medicine2005, (10): 780-782.
[1]姚玉梅,耿晓霞,吴亚云,程明亮.丹芍化纤胶囊对体内外活化肝星状细胞增殖的影响[J].中医杂志,2005(10):780-782+790. DOI： 10.13288/j.11-2166/r.2005.10.030.

Yao Yumei Geng Xiaoxia Wu Yayun. Effect of Danshao Huaxian Capsule on Proliferation of Activated Hepatic Stellate Cells in vivo and in vitro[J]. Journal of Traditional Chinese Medicine2005, (10): 780-782. DOI： 10.13288/j.11-2166/r.2005.10.030.

摘要

目的:研究中药复方制剂丹芍化纤胶囊对体内外活化肝星状细胞（HSCs）增殖的影响。方法:（1）体内实验:雄性 Wistar大鼠57只分为正常组、模型组、治疗组。除正常组外

其余各组采用 CCl

、饮酒、高脂低蛋白饮食等复合病因刺激制备肝纤维化动物模型8周

然后治疗组给予丹芍化纤胶囊（1g/kg体重）灌胃治疗8周。实验结束时部分大鼠用于测定肝功能及肝脏纤维化程度;部分用于分离 HSCs

流式细胞仪检测细胞周期百分比。（2）体外实验:制备丹芍化纤胶囊大鼠药物血清;分离培养大鼠 HSCs

并分为小牛血清组、正常大鼠血清组、丹芍化纤药物血清组

分别加入5%、10%、20%以上3种血清干预培养的HSCs

MTT 比色法测定原代 HSCs 增殖情况。结果:（1）体内实验:治疗组较模型组肝功能、肝脏纤维化程度及 HSCs 增殖明显减轻。（2）体外实验:丹芍化纤药物血清组较同浓度小牛血清组、正常大鼠血清组明显抑制 HSCs 增殖

且此作用呈剂量依赖性。结论:丹芍化纤胶囊能显著抑制体内外 HSCs 增殖。

Abstract

Objective:To study on effect of Danshao Huaxian Capsule on proliferation of activated hepatic stellate cells（HSCs）in vivo and in vitro.Methods:（1）In vitro experiment:57 male Wistar rats were divided into a normal group

a model group and a treatment group.The rats of model group and treatment group were made hepatic fibrosis model with complex stimulation of CCl

drinking alcohol

high lipids and low protein diet for 8 weeks.The treatment group were treated for 8 weeks with intragastric perfusion of Danshao Huaxian Capsule

1g/kg.At the end of experiment

a part of the rats were used for detection of hepatic functions and hepatic fibrosis degrees

and another part of the rats were used to separate HSCs.Cell cycle percentage was detected with flow cytometry.（2）In vivo experiment:prepare Danshao Huaxian Capsule serum of the rat;separate cultured HSCs of the rat and divide into calf serum group

normal rat serum group and Danshao Huaxian Capsule serum group.5%

10% and 20% of the above serum was respectively added into the cultured HSCs.Primary HSCs proliferation was detected with MTT method.Results:（1）In vitro experiment:The hepatic function

hepatic fibrosis level and HSCs proliferation reduced significantly.（2）In vivo experiment: Danshao Huaxian Capstde serum group could significantly inhibit proliferation of HSCs as compared with calf serum group and normal rat serum group at the same content.The action showed dose-dependence.Conclusion:Danshao Huaxian Capsule can obviously inhibit proliferation of HSCs in vivo and in vitro.

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