Serum Pharmacological Study on Relationship between Different Combinations of Banxia Xiexin Recipe and Inducing Apoptosis of BGC-823 Cells

Liu Xiping; Li Peiqing; Xi Shiyan; et al.

doi:10.13288/j.11-2166/r.2006.02.038

您当前的位置：

首页 >

文章列表页 >

Serum Pharmacological Study on Relationship between Different Combinations of Banxia Xiexin Recipe and Inducing Apoptosis of BGC-823 Cells

更新时间：2021-11-21

- Serum Pharmacological Study on Relationship between Different Combinations of Banxia Xiexin Recipe and Inducing Apoptosis of BGC-823 Cells
- Journal of Traditional Chinese Medicine Issue 2, Pages: 134-137(2006)
- 作者机构：
  
  1. 甘肃中医学院基础医学院方剂学教研室
  2. 甘肃省医学科学研究院药理毒理研究中心
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2006.02.038
  CLC： R285
- Published：2006
- 稿件说明：
移动端阅览
[1]刘喜平,李沛清,席时燕,李雪萍,梁涛.半夏泻心方配伍与诱导BGC-823细胞凋亡关系的血清药理学研究[J].中医杂志,2006(02):134-137.

Liu Xiping, Li Peiqing, Xi Shiyan, et al. Serum Pharmacological Study on Relationship between Different Combinations of Banxia Xiexin Recipe and Inducing Apoptosis of BGC-823 Cells[J]. Journal of Traditional Chinese Medicine2006, (2): 134-137.
[1]刘喜平,李沛清,席时燕,李雪萍,梁涛.半夏泻心方配伍与诱导BGC-823细胞凋亡关系的血清药理学研究[J].中医杂志,2006(02):134-137. DOI： 10.13288/j.11-2166/r.2006.02.038.

Liu Xiping, Li Peiqing, Xi Shiyan, et al. Serum Pharmacological Study on Relationship between Different Combinations of Banxia Xiexin Recipe and Inducing Apoptosis of BGC-823 Cells[J]. Journal of Traditional Chinese Medicine2006, (2): 134-137. DOI： 10.13288/j.11-2166/r.2006.02.038.

摘要

目的:研究半夏泻心方不同配伍药组含药血清对BGC-823细胞凋亡的影响及其机制。方法:采用MTT法观察半夏泻心方配伍药组含药血清对BGC-823细胞增殖的影响

采用DNA凝胶电泳检测细胞凋亡、S-P免疫组化法检测凋亡相关基因bcl-2的表达。结果:半夏泻心方配伍中辛开组、苦降组和由其组成的辛苦组可明显抑制BGC-823细胞的生长

显著优于辛甘组、苦甘组、甘补组和全方组

呈剂量-效应关系

其含药血清浓度在10%作用48小时后

DNA琼脂糖凝胶电泳可见较典型的“梯状带”

可使bcl-2基因的表达下调。结论:半夏泻心方中辛开、苦降的配伍形式具有抑制BGC-823细胞增殖

诱导细胞凋亡作用

辛开、苦降配伍后（辛苦）有显著的协同增效趋势

而其他配伍药群可能存在拮抗作用。提示辛开苦降法可能是中医药防治胃癌的主要治则治法之一。

Abstract

Objective:To study the effects of medicine-containing serum for different combinations of Banxia Xiexin Recipe on BGC-823 cell apoptosis and its mechanisms.Methods:MTT method was used to observe effects of medicine-containing serum for different combinations of Banxia Xiexin Recipe on proliferation of BGC-823 cells

DNA gel electrophoresis used to detect cellular apoptosis

and S-P immunohistochemical method used to investigate expression of apoptosis-related gene bcl-2.Results:Growth of BGC-823 cells was significantly inhibited in the pungent-opening group

the bitter-descending group and the pungent-bitter group

which was significantly higher than that in the pungent-sweet group

bitter-sweet group

the sweet-invigorating group and the complete recipe group（P<0.05）

with a dose-effect relation.After action of 10% medicine-containing serum for 48 hours

a more typical “scalariform band” could be seen on the DNA agar gel electrophoresis

and bcl-2 gene expression was down-regulated.Conclusion:The combination of pungent-opening or bitter-descending components in the Banxia Xiexin Recipe has the actions of inhibiting proliferation of BGC-823 cells and inducing cell apoptosis

and after the combination of pungent-opening or bitter-descending components（Pungent-bitter） has a significant tendency to cooperatively increase the effect

but other combinations maybe have antagonism.It is suggested that pungent-opening and bitter-descending method is possibly one of main therapeutic principles and methods for prevention and treatment of gastric cancer with TCM.

关键词

Keywords

references

查看原文

Views

303

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Syndrome Differentiation and Treatment of Cholinergic Urticaria from the Perspective of "Treating Fire Constraint by Dispersing It"

Current Status and Strategic Recommendations for Randomized Controlled Trials on Functional Dyspepsia-Irritable Bowel Syndrome Comorbidity：from a Real-World Research Perspective

Clinical Efficacy and Economic Evaluation of 1293 Non-Severe Adult Patients with Community-Acquired Pneumonia Treated by the Jiangsu Traditional Chinese Medicine Diagnosis and Treatment Protocol for Dominant Diseases：A Multicenter，Retrospective Real-World Cohort Study

Exploration on the Connotation and Application of the Three Aspects of "Dietary Inquiry" for "Appetite" "Eating Capacity" "Food Preference"

Exploring the Comorbidity Mechanism，Prevention and Treatment Strategies of Rheumatoid Arthritis with Coronary Atherosclerotic Heart Disease Based on Collateral Disease Theory

Related Author

No data

Related Institution

No data

Address：No.16, Nanxiao street, Dongzhimen, Dongcheng District, Beijing Postal code：100700
Tel：010-64035632 Email：jtcmcn@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010102007558号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰