Study on Differential Expression of Proteome in the Liver Tissue of the Model Rat with Stagnation of the Liver-qi Induced by Restraint

Zhong Xiaolan~1; Lv Zhiping~1; Qian Lingjia~2; et al.

doi:10.13288/j.11-2166/r.2006.05.031

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Study on Differential Expression of Proteome in the Liver Tissue of the Model Rat with Stagnation of the Liver-qi Induced by Restraint

更新时间：2021-11-20

- Study on Differential Expression of Proteome in the Liver Tissue of the Model Rat with Stagnation of the Liver-qi Induced by Restraint
- Journal of Traditional Chinese Medicine Issue 5, Pages: 371-373(2006)
- 作者机构：
  
  1. 南方医科大学中医药学院
  2. 军事医学科学院卫生学环境医学研究所
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2006.05.031
  CLC： R-332
- Published：2006
- 稿件说明：
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[1]钟小兰,吕志平,钱令嘉,刘晓华,谭秦湘,张晓刚.束缚所致肝郁证动物模型肝组织蛋白质组的差异表达研究[J].中医杂志,2006(05):371-373.

Zhong Xiaolan~1, Lv Zhiping~1, Qian Lingjia~2, et al. Study on Differential Expression of Proteome in the Liver Tissue of the Model Rat with Stagnation of the Liver-qi Induced by Restraint[J]. Journal of Traditional Chinese Medicine2006, (5): 371-373.
[1]钟小兰,吕志平,钱令嘉,刘晓华,谭秦湘,张晓刚.束缚所致肝郁证动物模型肝组织蛋白质组的差异表达研究[J].中医杂志,2006(05):371-373. DOI： 10.13288/j.11-2166/r.2006.05.031.

Zhong Xiaolan~1, Lv Zhiping~1, Qian Lingjia~2, et al. Study on Differential Expression of Proteome in the Liver Tissue of the Model Rat with Stagnation of the Liver-qi Induced by Restraint[J]. Journal of Traditional Chinese Medicine2006, (5): 371-373. DOI： 10.13288/j.11-2166/r.2006.05.031.

摘要

目的:通过运用组织蛋白质组学技术和方法

寻找肝郁证动物模型肝组织差异表达蛋白质。方法:以束缚制动法制备肝郁证大鼠模型

采用组织溶解方法分步抽提大鼠肝脏组织蛋白质

对获得的蛋白质进行二维凝胶电泳（2-DE）分离、胶体染色

分析模型组和对照组蛋白质分子的差异表达

并以基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）检测肽质量指纹

在数据库中检索出相应的蛋白质。结果:在大鼠肝组织胶体考染的2-DE图谱上

约能辨识出300余个清晰的蛋白质斑点

共找出3个与肝郁证辨证相关的肝组织差异表达蛋白

质谱出峰率达100%

检索结果分别为芳基硫基转移酶、烯酰辅酶A水合酶和转甲状腺素蛋白。结论:肝主疏泄功能的正常可能和实体肝组织中的特异表达蛋白质——芳基硫基转移酶、烯酰辅酶A水合酶和转甲状腺素蛋白的不同表达密切相关。

Abstract

Objective:To search for differentially expressed proteins in the liver tissue of the model rat with stagnation of the liver-qi by using technique and method of tissue proteomics.Methods:The rat model with stagnation of the liver-qi was established by restraint.Proteins in the liver tissue of the rat were extracted with histolysis step by step

and the attained proteins were separated by two-dimensional electrophoresis（2-DE）

with colloidal staining.The differential depressions of proteins were analyzed in the model group and the control group.The peptide mass fingerprints were detected with MALDI-TOF-MS

and the corresponding proteins were retrieved in Databank.Results:In the colloidal stained 2-DE map of the liver tissue of the rat

over 300 clear protein spots could be differentiated

and totally 3 differential expressed proteins in the liver tissue related with syndrome of stagnation of the liver-qi were found

the mass-spectrogram peaking rate being 100%.They were aryl sulfotransferase

enoyl-coa hydrase and transthyretin.Conclusion:The normal dispelling function of the liver is possibly related with differential expression of the above 3 transpotanted proteins.

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