Objective:To observe effect of recipe or herbs of invigorating the spleen on gastrin receptor and intracellular calcium ion [Ca

2+ ]i level of parietal cells in spleen deficiency model rats.Methods:The parietal cells of the rats were isolated by EDTA-Pronase method.Gastrin receptor was detected with radioligand binding assay and intracellular calcium ion level was determined with double wave-length fluorescence method.Results:The gastrin receptor binding sites of parietal cells in the model group were significantly decreased （P

<

0.01）

and they were increased after treatment by the herbs or recipe of invigorating the spleen.The intracelluler resting [Ca

2+ ]i of parietal cells in the model group was significantly lower than that in the normal group（P

<

0.01）

and it increased after stimulation of gastrin.The increasing amplitude in the model group and the Dangshen group was significantly higher than that in the normal group（P

<

0.05）

and after treatment the condition was not significantly changed.Conclusion:Spleen deficiency syndrome is possibly related with low expression of gastrin receptor

and calcium ion of parietal cells is at low level.But the reactivity to gastrin stimulation was strengthened

possibly further inducing relative increase of sensitivity of acid secretion and injury of gastric mucosa

on which the medical herbs or recipe of invigorating the spleen have regulative action.