Study on Characteristics of Peripheral Blood Gene Expression Profile in the Obesity with Phlegm-Dampness Constitution

doi:10.13288/j.11-2166/r.2006.11.030

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Study on Characteristics of Peripheral Blood Gene Expression Profile in the Obesity with Phlegm-Dampness Constitution

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- Study on Characteristics of Peripheral Blood Gene Expression Profile in the Obesity with Phlegm-Dampness Constitution
- Journal of Traditional Chinese Medicine Issue 11, Pages: 851-853(2006)
- 作者机构：
  
  1. 北京中医药大学体质与生殖医学研究中心
  2. 中国人民解放军武装警察部队北京市总队医院北京市朝阳区北三环东路11号,100029
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2006.11.030
  CLC： R259
- Published：2006
- 稿件说明：
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[1]王琦,龚海洋,高京宏.肥胖人痰湿体质外周血基因表达谱特征研究[J].中医杂志,2006(11):851-853+858.

Study on Characteristics of Peripheral Blood Gene Expression Profile in the Obesity with Phlegm-Dampness Constitution[J]. Journal of Traditional Chinese Medicine2006, (11): 851-853.
[1]王琦,龚海洋,高京宏.肥胖人痰湿体质外周血基因表达谱特征研究[J].中医杂志,2006(11):851-853+858. DOI： 10.13288/j.11-2166/r.2006.11.030.

Study on Characteristics of Peripheral Blood Gene Expression Profile in the Obesity with Phlegm-Dampness Constitution[J]. Journal of Traditional Chinese Medicine2006, (11): 851-853. DOI： 10.13288/j.11-2166/r.2006.11.030.

摘要

目的采用基因芯片技术对痰湿型体质人的外周血基因表达谱进行研究。方法挑选3例非痰湿型体质肥胖人、2例痰湿型体质肥胖人和3例正常体重者

取上述受试者的外周血

提取总RNA并进行扩增和标记

检测受试样品的质量

进行基因表达谱观察

从基因序列中查出具有显著统计学意义的序列所标志的基因。结果痰湿型体质与非痰湿型体质相比

有115个差异表达探针组。结论本研究发现痰湿型体质人的外周血相关基因表达与非痰湿型体质人有明显差异。

Abstract

Objective:To study on peripheral blood gene expression profile in the obesity with phlegm-dampness constitution by gene chip technique.Methods:Three cases of non-phlegm-dampness constitution

2 cases of phlegm-dampness constitution

and 3 normal persons were selected and the extracted total RNA was magnified and labeled.Then the quality of samples was detected and the gene expression profile was investigated.The genes marked by the sequence with statistically significant difference were searched out from Gene Bank.Results:As the phlegm-dampness constitution compared with the non-phlegm-dampness constitution

there are 115 probe groups of differential expression.Conclusion:There were significant differences of peripheral blood relative genes expression between the phlegm-dampness constitution and the non-phlegm-dampness constitution.

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