Effect of Tiaogan Jiedu Formula on Learning and Memory in Rats with Chronic Epilepsy and Expression of cAMP Responsive Element Binding Protein in Hippocampus

PEI Lin1; LI Dian-gui1; LOU Yan2; et al.

doi:10.13288/j.11-2166/r.2007.03.041

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Effect of Tiaogan Jiedu Formula on Learning and Memory in Rats with Chronic Epilepsy and Expression of cAMP Responsive Element Binding Protein in Hippocampus

更新时间：2021-11-17

- Effect of Tiaogan Jiedu Formula on Learning and Memory in Rats with Chronic Epilepsy and Expression of cAMP Responsive Element Binding Protein in Hippocampus
- Journal of Traditional Chinese Medicine Issue 3, Pages: 265-268(2007)
- 作者机构：
  
  1. 河北医科大学中医药研究院
  2. 河北省人民医院
  3. 河北医科大学基础医学研究所河北省石家庄市建华南大街209号
  4. ,050031
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2007.03.041
  CLC： R285.5
- Published：2007
- 稿件说明：
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[1]裴林,李佃贵,娄燕,杨雁,吴国群.调肝解毒方对慢性癫痫大鼠学习记忆及海马组织环磷酸腺苷反应元件结合蛋白表达的影响[J].中医杂志,2007(03):265-268.

PEI Lin1, LI Dian-gui1, LOU Yan2, et al. Effect of Tiaogan Jiedu Formula on Learning and Memory in Rats with Chronic Epilepsy and Expression of cAMP Responsive Element Binding Protein in Hippocampus[J]. Journal of Traditional Chinese Medicine2007, (3): 265-268.
[1]裴林,李佃贵,娄燕,杨雁,吴国群.调肝解毒方对慢性癫痫大鼠学习记忆及海马组织环磷酸腺苷反应元件结合蛋白表达的影响[J].中医杂志,2007(03):265-268. DOI： 10.13288/j.11-2166/r.2007.03.041.

PEI Lin1, LI Dian-gui1, LOU Yan2, et al. Effect of Tiaogan Jiedu Formula on Learning and Memory in Rats with Chronic Epilepsy and Expression of cAMP Responsive Element Binding Protein in Hippocampus[J]. Journal of Traditional Chinese Medicine2007, (3): 265-268. DOI： 10.13288/j.11-2166/r.2007.03.041.

摘要

目的观察调肝解毒方对慢性癫痫大鼠学习记忆与海马组织环磷酸腺苷反应元件结合蛋白（CREB）表达的影响。方法60只大鼠腹腔注射印防己毒素制作慢性癫痫模型后

随机分为模型组

调肝解毒方低剂量组、调肝解毒方高剂量组

丙戊酸钠组

每组15只

与正常组（15只）同期观察或治疗60天

以Morris水迷宫观察大鼠空间学习和记忆能力

应用RT-PCR方法检测大鼠海马组织CREBmRNA表达变化

免疫组织化学方法检测海马组织磷酸化的CREB水平变化。结果调肝解毒方低、高剂量组大鼠空间记忆能力高于模型组（P<0.01）

海马组织CREBmRNA相对吸光度值增高

pCREB免疫阳性细胞的表达有不同程度增强

与模型组比较差异有显著性意义（P<0.05

P<0.01）。结论调肝解毒方可在一定程度上改善反复癫痫大鼠的学习记忆能力

可能与调节海马CREB表达有关。

Abstract

Objective To study the effect of Tiaogan Jiedu Formula（TJF） on learning and memory in rats with chronic epilepsy and expression of cAMP responsive element binding protein（CREB） in hippocampus.Methods Totally 60 chronic epilepsy model rats were randomly and equally divided into model group

TJF large-dose group

TJF small-dose group and Sodium Valproate group after intraperitoneal injection of Picrotoxin.The observation and treatment lasted for 60 days

and normal group of 15 rats were observed simultameously .The ability of learning and memory was tested by Morris water maze.The expression of CREB mRNA in hippocampus and level of phosphorylated CREB in hippocampus were detected by reverse transcription polymerase chain reaction and immunohistochemical method respectively.Results Memory ability

relative absorbance of CREB mRNA and positive expression of CREB immunocytes in TJF large-dose and small-dose groups increased significantly as compared with model group（P<0.01 or P<0.05）.Conclusion TJF can improve learning and memory ability of rats with chronic epilepsy

which might be relevant to its regulating effect on the expression of CREB in hippocampus.

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Related Institution

北京中医药大学东直门医院

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First Affiliated Hospital,Tianjin University of TCM

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