Effect of Different Combinations of Sanchinoside Monomers on Monocyte-Endotheliocyte Adhesion

HE Cui-yao; LI Xiao-hui; HE Xue-feng

doi:10.13288/j.11-2166/r.2007.04.036

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Effect of Different Combinations of Sanchinoside Monomers on Monocyte-Endotheliocyte Adhesion

更新时间：2021-11-17

- Effect of Different Combinations of Sanchinoside Monomers on Monocyte-Endotheliocyte Adhesion
- Journal of Traditional Chinese Medicine Issue 4, Pages: 354-356(2007)
- 作者机构：
  
  1. 第三军医大学基础部新药研究室
  2. 第三军医大学基础部新药研究室重庆市沙坪坝区高滩岩正街30号
  3. ,400038
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2007.04.036
  CLC： R285.5
- Published：2007
- 稿件说明：
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[1]何翠瑶,李晓辉,何雪峰.三七皂苷单体不同配伍对单核-内皮细胞黏附作用的影响[J].中医杂志,2007(04):354-356+361.

HE Cui-yao, LI Xiao-hui, HE Xue-feng. Effect of Different Combinations of Sanchinoside Monomers on Monocyte-Endotheliocyte Adhesion[J]. Journal of Traditional Chinese Medicine2007, (4): 354-356.
[1]何翠瑶,李晓辉,何雪峰.三七皂苷单体不同配伍对单核-内皮细胞黏附作用的影响[J].中医杂志,2007(04):354-356+361. DOI： 10.13288/j.11-2166/r.2007.04.036.

HE Cui-yao, LI Xiao-hui, HE Xue-feng. Effect of Different Combinations of Sanchinoside Monomers on Monocyte-Endotheliocyte Adhesion[J]. Journal of Traditional Chinese Medicine2007, (4): 354-356. DOI： 10.13288/j.11-2166/r.2007.04.036.

摘要

目的研究三七皂苷不同单体及单体间两两配伍对人单核-内皮细胞黏附的影响及机制。方法三七皂苷单体及不同单体配伍处理内皮细胞后

加入氧化低密度脂蛋白（ox-LDL）

以孟加拉玫瑰红活细胞染色法测定人单核细胞系THP-1与人脐静脉内皮细胞（HUVECs）的黏附

并用ELISA法测定内皮细胞黏附分子（ICAM-1）在HUVECs的表达水平。结果ox-LDL显著增强THP-1与HUVECs的黏附

内皮细胞经单体Rg1、Rb1处理后

明显抑制ox-LDL诱导的单核-内皮细胞黏附及ICAM-1在HUVECs的表达（P<0.05）

Rg1:Rb1、Rg1:R1、Rb1:R1配伍组对单核-内皮细胞黏附的抑制作用更为显著

并能显著下调ICAM-1的表达（P<0.01）。结论（1）三七皂苷单体Rg1、Rb1为抑制黏附的有效单体

单体Rg1、Rb1、R1之间存在某种协同作用

对ox-LDL诱导的单核-内皮细胞黏附发挥更显著抑制作用。（2）单体Rg1、Rb1及有效单体配伍组合可能通过下调ICAM-1的表达抑制单核-内皮细胞黏附

从而发挥血管内皮细胞的保护作用。

Abstract

Objective To study the effect and mechanism of different combinations of sanchinoside monomers on monocyte-endotheliocyte adhesion.Methods Oxidized low density lipoprotein（ox-LDL）was added to endotheliocytes processed by sanchinoside monomers and their different combinations.Then rose bengal staining was used to detect adhesion of human THP-1 monocytes to human umbilical vein endothelial cells（HUVECs）

and enzymelinked immunosorbent assay was used to detect the expression of intercellular adhesion molecule-1（ICAM-1）.Results Ox-LDL could enhance adhesion of THP-1 to HUVECs.After processed by Rg1 and Rb1

monocyte-endotheliocyte adhesion and expression of ICAM-1 decreased significantly（P<0.05）

especially in combination groups such as Rg1:Rb1

Rg1:R1

and Rb1:R1（P<0.01）.Conclusion Rg1 and Rb1 can obviously inhibit moncyte-endotheliocyte adhesion induced by ox-LDL

especially in different combinations of Rg1

Rb1 and R1.Rg1

Rb1 and their combinations could inhibit monocyte-endotheliocyte adhesion by down-regulating the expression of ICAM-1

which might be related to its protection effect on endotheliocytes.

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