Objective To study the lymphocyte NF-к B signal passage cross membrane acceptor mRNA expression characteristics of rats in increasing age and the intervention effect of total flavone of Epimedium（EF）.Methods The SD rats were randomized into 6 groups: 3 days（3d）

4 months（4m）

10 months（10m）

18 months（18m）

27 months（27m）

27m+EF.The lymphocytes separated from the spleen of rats were taken as the object and the NF-кB signal passage gene chip was used to examine the expression characteristics and differences of NF-кB signal passage upstream cross membrane acceptor mRNA of each group and to observe intervention effect of EF on NF-κB related gene.Results The expression of Tgfbr2

Tlr7

Ripk2 of 3d was the lowest

reached the peak 4m or 10m

afterward gradually lowered

dropping to the lowest 27m

with EF intervention

Ripk2 changed obviously

close to 10m level;Tnfrsf1a and Tlr2 reached the peak on the 3d

in the 4m reduced suddenly

then in 10m

18m and 27m maintained at a similar level

but with the EF intervention they were obviously increased;Tnfrsf5 increased gradually from 3d to 18m

but dropped suddenly in 27m

with EF intervention

it was slightly increased;the Madh4 expression was quite special

to the highest in 4m

to the lowest in 10m

increased obviously in 18m

reduced again in 27m

with EF intervention

slightly increased;the change of Tnfrsf10b

Il1r1

Tlr1

Tlr4 in increasing age was not obvious.Conclusion In the senile advancement

lymphocyte NF-κB signal passage cross membrane acceptor mRNA increasing age dependence expression regulation characteristics were not inconsistent

the EF intervention produces a certain good influence on the old age group for gene mRNA expression

particularly a remarkable effect on Ripk2.This is possibly one of important targets of EF functions.