Objective To explore the antagonistic effect of Ziyin Xiehuo Recipe（ZYXHR

Recipe for nourishing kidney yin and purging ministerial fire） on estrogen-like activity of environmental endocrine disruptors

nonylphenol（NP） and mixture of NP and bisphenol A（BPA）. Methods Totally 30 three-week-old female SD rats were randomly divided into control group（fed with corn oil）

exposed group A （feeding NP100 mg / kg ）

exposed group B （feeding NP 50 mg/kg+ BPA 200 mg/kg ）

treatment group A （NP 100 mg/kg + ZYXHR）

treatment group B （NP 50 mg/kg+ BPA 200 mg/kg + ZYXHR）. After 15 days of treatment

the uterine weight gain and PCNA protein expression were detected. Results As compared with those of the control group

Uterine wet weight

uterus organ coefficient

thickness of endometrium and smooth muscle

and the height of endometrial glands and glandular epithelium significantly increased in exposed group A and B （P<0.05）

PCNA expression in endometrium and myometrium significantly increased as well （P<0.05）. Above indictors were significant decreased in treatment group A and B as compared with those in exposed group A and B （P<0.05）. Conclusion NP and BPA have estrogenic-like activity

ZYXHR can be antagonistic to the estrogenic effect of NP and BPA.