Objective To find out the optimized parameter （frequency and time） of the main acupoint Shuigou （GV 26） in Acupuncture for Restoring Consciousness and Inducing Resuscitation for the brain infarction treatment. Methods The 132 SD rats were randomized into sham operation group

model untreated group and model treated group. The model treated group was subdivided into 9 groups according to the needling time （5

60 and 180 seconds） and frequency （1

2

and 3 times per second）. The ischemia models of middle cerebral artery （MCA） on left side in the model groups were made. The model treated group was given acupuncture at Shuigou （GV 26） with 9 kinds of parameters 3 hours after the MCA occlusion and every 12 hours there-after

totally 6 time points. Then

the evaluation on neuroethology

the calculation on volume of blood flow and microcirculation of pia mater

measuring the ischemia area and observation on brain tissues with light microscope were performed. The acquired data based on the grey weight relation was analyzed to find out the optimized parameters of Shuigou （GV 26） for the treatment of brain infarction. Results The grey relation degrade of parameters was listed as: parameter 6>parameter 1>parameter 2>parameter 9>parameter 7>parameter 4>parameter 3>parameter 8>parameter 5. The parameter 6 （Frequency: 3 times/s

Time: 5 seconds） could increase the volume of blood flow

enlarge the diameter of vessel

decrease the ischemia area

and reduce the number of positive cells and neurophages. Conclusion The optimized parameter of Shuigou （GV 26） for the treatment of brain infarction is 3 times/s and 5 seconds.