Objective To observe the mechanism of Xiongqi Mixture（Mixture of Rhizome Chuanxiong and Radix Astragali） resisting cerebral ischemical reperfusion injury after thrombolysis from the aspects of excitatory amino acid and metabonomics.Methods The middle cerebral artery occlusion（MCAO） models of SD rats were set up and reperfusion was carried out after thrombolysis.The rats were randomized into normal control group

physiological saline control group

urekinase treatment group

Xiongqi Mixture treatment group

and urekinase + Xiongqi Mixture treatment group

with 30 rats in each group.The histological biochemistry and NMR-based metabonomics methods were used for detecting the excitatory amino acid and plasma metabonomics indexes.Results Xiongqi Mixture could inhibit the increase of Glu and Asp caused by reperfusion of MCAO rats with cerebral ischemia（P <0.05）

making them tend to normal.The metabonomics detection showed that Xiongqi Mixture could inhibit the increase of plasma glutamate

lactate

creatine

choline and the decrease of threonine

alanine and blood glucose metablism（P<0.05）.Conclusion Xiongqi Mixture can regulate the metabolism of MCAO rats with cerebral ischemia after reperfusion from the diseased condition

resisting the reperfusion injury.