Objective To explore the relationship between gene mutation and different syndromes of TCM in the early stage of diabetic nephropathy（DN）.Methods Sixty-three patients with diabetic nephropathy in the early stage were observed.The methylene tetrahydrofolate reductase（MTHFR）C676T polymorphism was determined by polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP）assay.The levels of homocysteine（Hcy）

acidum folicum

fasting glucose

postprandial glucose

glycosylated hemoglobin（HbA1C）

urinary albumin excretion rate（UAER）

and blood lipid were measured respectively

and the TCM syndromes were recorded.Results Of the 63 patients

19 were genotype CC

17 were genotype TT

and 27 were genotype CT.The genotypic frequency of TT was 27.00%

that of CT was 42.85%

and that of CC was 30.15%.The T allele frequency was 48.41% and C allele frequency 51.59%.The MTHFR C676T mutation was related with plasma Hcy level（P<0.01）and triglyceride（TG）level（P<0.05）.TCM syndromes were related with genotypes（P<0.01）.The genotype CC was mostly in the syndrome of yin deficiency with heat

the genotype CT was mostly in the syndrome of both qi and yin deficiency

and the genotype TT was mostly in the syndrome of both yin and yang deficiency.Conclusion The MTHFR C677 base alkali polymorphism in patient of early DN was related with plasma Hcy and TG levels and TCM syndromes.