Objective To investigate the protective effect and mechanism of Ronggan Mixture（Herbal Decoction for liver diseases）on the mice model with concanavalin A-induced acute immunological liver injury.Methods Totally 60 HBV transgenic mice were randomized into model group

control group

Ronggan Mixture group

Yinchenhao Decoction group

Yinchen group

and Bifendate group

with 10 in each.The acute immunological liver injury models were established by the tail vein injection of concanavalin A.Fourteen days before modeling

the model group and control group were given normal saline by gavage

while the other groups were treated with Ronggan Mixture

Yinchenhao Decoction（Oriental Wormwood Decoction）

Yinchen（Virgate Wormwood Herb）

and Bifendate respectively by gavage everyday.One hour after the last intervention

the control group was given phosphate buffer solution（PBS）by the tail vein injection

and the others were given concanavalin A by the tail vein injection to establish models.Eight hours after modeling

the mice were sacrificed

the serum and tissue samples were collected to detect:alanine aminotransferase（ALT）

aspartate aminotransferase（AST）

total bilirubin（TBiL）

hepatitis B virus quantity（HBV DNA）

hepatitis B surface antigen（HBsAg）

superoxide dismutase（SOD）

and malondialdehyde（MDA）.Results Compared with the model group and the others

Ronggan Mixture could decrease the level of ALT

AST

and TBiL（P<0.05 or P<0.01）.Compared with the Bifendate group

Yinchen group

and Yinchenhao Decoction group

Ronggan Mixture could decrease the HBV DNA and the MDA of liver tissues

and increase SOD level of liver tissues.Conclusion Ronggan Mixture is of advantage in decreasing the enzymes as compared with Yinchen

Yinchenhao Decoction and Bifendate.The liver protective effect is achieved through inhibiting HBV DNA to reduce the injury of the lipid peroxidation of hepatocyte membrane.