Effect of Artemisia Argyi Extracting Solution Contained Rat Serum on Eexpression of Transforming Growth Factor-β<sub>1</sub> and Protein Smads in Hepatic Stellate Cells

XIONG Zhenfang1; WANG Weimin1; FEI Xinying1; ZHANG Chizhi2

doi:10.13288/j.11-2166/r.2010.06.037

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Effect of Artemisia Argyi Extracting Solution Contained Rat Serum on Eexpression of Transforming Growth Factor-β₁ and Protein Smads in Hepatic Stellate Cells

更新时间：2021-11-17

- Effect of Artemisia Argyi Extracting Solution Contained Rat Serum on Eexpression of Transforming Growth Factor-β₁ and Protein Smads in Hepatic Stellate Cells
- Journal of Traditional Chinese Medicine Vol. 51, Issue 6, Pages: 554-556(2010)
- 作者机构：
  
  1. 湖北中医药大学
  2. 湖北省中医院感染科
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2010.06.037
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]熊振芳,王伟民,费新应,张赤志.蕲艾提取液含药血清对肝星状细胞转化生长因子β_1及Smad蛋白表达的影响[J].中医杂志,2010,51(06):554-556.

XIONG Zhenfang1, WANG Weimin1, FEI Xinying1, et al. Effect of Artemisia Argyi Extracting Solution Contained Rat Serum on Eexpression of Transforming Growth Factor-β₁ and Protein Smads in Hepatic Stellate Cells[J]. Journal of Traditional Chinese Medicine2010, 51(6): 554-556. DOI: 10.13288/j.11-2166/r.2010.06.037.
[1]熊振芳,王伟民,费新应,张赤志.蕲艾提取液含药血清对肝星状细胞转化生长因子β_1及Smad蛋白表达的影响[J].中医杂志,2010,51(06):554-556. DOI： 10.13288/j.11-2166/r.2010.06.037.

XIONG Zhenfang1, WANG Weimin1, FEI Xinying1, et al. Effect of Artemisia Argyi Extracting Solution Contained Rat Serum on Eexpression of Transforming Growth Factor-β₁ and Protein Smads in Hepatic Stellate Cells[J]. Journal of Traditional Chinese Medicine2010, 51(6): 554-556. DOI: 10.13288/j.11-2166/r.2010.06.037. DOI：

摘要

目的探讨蕲艾提取液的抗肝纤维化作用及其机制。方法 24只Wistar大鼠随机分为蕲艾组、丹参组、生理盐水组和空白组

每组6只。其中

蕲艾组用20%的蕲艾提取液灌胃

丹参组用丹参注射液灌胃

3组灌液量均为20ml/kg

每天3次

连续3天

末次给药1h后采血取血清

用细胞培养液DMEM配制成体积分数分别为5%、10%和20%的含药培养液。分别检测各体积分数的蕲艾提取液含药培养液对HSC/T6细胞转化生长因子（TGF-β1）、Smad3、Smad7mRNA和蛋白的影响。结果 5%、10%和20%的蕲艾提取液含药血清培养HSC/T6细胞24h后TGF-β1mRNA表达水平分别为空白组的（0.82±0.03）、（0.72±0.03）和（0.67±0.04）倍

蛋白表达水平分别为（0.66±0.09）、（0.34±0.05）和（0.31±0.07）倍

与生理盐水组相比显著降低（P<0.05）;且均下调Smad3mRNA和蛋白水平

同时上调Smad7mRNA和蛋白表达。蕲艾提取液含药血清20%组与丹参组相比无统计学差异。结论蕲艾提取液含药血清抑制肝星状细胞TGF-β1和Smad3的表达

上调Smad7的表达

从而抑制HSC/T6活化、增殖

降低胶原的表达及分泌

达到抗纤维化的目的。

Abstract

Objective To investigate the effect and mechanism of Artemisia Argyi extracting solution on anti-hepatic fibrosis.Methods Twenty-four experimental Wistar rats were randomized into four groups:Artemisia group

Danshen group

physiological saline group

and blank control group

with 6 in each.The Artemisia group was treated with 20% Artemisia Argyi extracting solution

the Danshen group with Danshen （salvia） solution

and the physiological saline group with physiological saline by gavage

20ml/kg.After treating three times per day for 3 days

sera were obtained and made a mixture with DMEM to be volume fraction as 5%

10%

and 20%.Effect of different volume fraction of extracting solution on TGF-β1 Smad3 and Smad7 mRNA and protein were detected.Results After treating with 5%

10% and 20% Artemisia Argyi extracting solution contained rat serum for 24h

contrasted to the black control group

the expression of TGF-β1 mRNA level was （0.82±0.03）

（0.72±0.03） and （0.67±0.04） times

and the expression of TGF-β1 protein was （0.66±0.09）

（0.34±0.05） and （0.31±0.07） times respectively;contrasted to the physiological saline group

the expressions of TGF-β1 mRNA and protein were significantly decreased （P<0.05）

and the expressions of Smad3 mRNA and protein were down-regulated and at the same time the expressions of Smad7 mRNA and protein were up-regulated.There was no significant difference between the 20% Artemisia Argyi extracting solution contained rat serum group and the Danshen group.Conclusion Artemisia Argyi extracting solution contained rat serum plays a role in anti-fibrosis by the way of reducing TGF-β1 and Smad3 and up-regulating Smad7

which leads to inhibiting the activation and proliferation of HSC-T6 to reduce the collagen expression and secretion.

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Effect of Artemisia Argyi Extracting Solution Contained Rat Serum on Eexpression of Transforming Growth Factor-β1 and Protein Smads in Hepatic Stellate Cells

DOI：10.13288/j.11-2166/r.2010.06.037

摘要

Abstract

关键词

Keywords

references

Effect of Artemisia Argyi Extracting Solution Contained Rat Serum on Eexpression of Transforming Growth Factor-β₁ and Protein Smads in Hepatic Stellate Cells