Study on Making the Animal Model of Diarrhea-predominant Irritable Bowel Syndrome Due to Liver-Spleen Disharmony

HU Rui; TANG Fang

doi:10.13288/j.11-2166/r.2010.06.040

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Study on Making the Animal Model of Diarrhea-predominant Irritable Bowel Syndrome Due to Liver-Spleen Disharmony

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- Study on Making the Animal Model of Diarrhea-predominant Irritable Bowel Syndrome Due to Liver-Spleen Disharmony
- Journal of Traditional Chinese Medicine Vol. 51, Issue 6, Pages: 547-550(2010)
- 作者机构：
  
  天津医科大学总医院中医科
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2010.06.040
  CLC： R-332
- Published：2010
- 稿件说明：
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[1]胡瑞,唐方.肝脾不调所致腹泻型肠易激综合征的动物模型研究[J].中医杂志,2010,51(06):547-550.

HU Rui, TANG Fang. Study on Making the Animal Model of Diarrhea-predominant Irritable Bowel Syndrome Due to Liver-Spleen Disharmony[J]. Journal of Traditional Chinese Medicine2010, 51(6): 547-550.
[1]胡瑞,唐方.肝脾不调所致腹泻型肠易激综合征的动物模型研究[J].中医杂志,2010,51(06):547-550. DOI： 10.13288/j.11-2166/r.2010.06.040.

HU Rui, TANG Fang. Study on Making the Animal Model of Diarrhea-predominant Irritable Bowel Syndrome Due to Liver-Spleen Disharmony[J]. Journal of Traditional Chinese Medicine2010, 51(6): 547-550. DOI： 10.13288/j.11-2166/r.2010.06.040.

摘要

目的探讨肝脾不调型腹泻型肠易激综合征（IBS-D）动物模型的制备方法。方法为确定造模周期

32只乳鼠分为模型3天组、6天组、9天组和空白对照组;为确定实验动物体重

48只大鼠按体重分层

随机分为高、中、低体重模型组和相应空白对照组;为确定致泻方法

32只大鼠分为高乳糖组、应激组、高乳糖加应激组和空白对照组

分别比较相关各组大鼠体重、腹泻情况。确定造模方法后

16只大鼠分为模型组和空白对照组

检测5-羟色胺（5-HT）、乳酸脱氢酶（LDH）及水通道蛋白（AQP4）验证模型。结果依据造模周期实验、确定动物体重实验及不同致泻方法实验的结果

确定最佳造模方法为:实验周期6天

选取高体重大鼠为实验对象

采用高乳糖饲料加束缚应激联合致泻。模型验证实验:与空白对照组比较

模型组5-HT及LDH下降（P<0.05或P<0.01）

AQP4升高（P<0.05）。结论该模型模拟临床IBS以胃肠症状为主要表现、多与情绪相关的特点

具备IBS-D病理改变

可用于相关药物的实验研究。

Abstract

Objective To explore the animal model making method for diarrhea-predominant irritable bowel syndrome （IBS-D） caused by the disharmony of liver and spleen in TCM.Methods In order to define the period of the animal model making

the 32 neonatal rats were divided into 3 days group

6 days group

9 days group and control group.In order to determine the weight of the experimental rats

the 48 rats with different weight were divided into lower weight model group

lower weight control group

medium weight model group

medium weight control group

higher weight model group and higher weight control group.In order to decide the method of causing diarrhea

the 32 rats were divided into lactose enriched diet group

restraint stress group

lactose enriched diet plus restraint stress group

and a control group.The body weight and diarrhea rate of all groups were compared respectively.After the method of model making was decided

the 16 rats were divided into a model group and a control group

and 5-hydroxytryptamin （5-HT）

lactate dehydrogenase （LDH） and aquaporin 4 （AQP4） of the two groups were compared for model-proving.Results Based on the results step by step

the best method was decided:the period of the animal model making was 6 days

rats with higher body weight should be subjects

and the lactose enriched diet combining with restraint stress should be used to induce diarrhea.Model-proving test:compared with the control group

5-HT and LDH decreased （P<0.01

P<0.05） and AQP4 increased （P<0.05） in the model group.Conclusion The animal model has the symptoms of gastrointestinal system

and its pathological changes relate to emotion.So it can be used in the experimental study of IBS.

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Related Institution

TCM Hospital of Wuhan City

Hubei University of Traditional Chinese Medicine

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Anyue TCM Hospital of Sichuan Province

College of Preclinical Medicine,Henan University of Chinese Medicine

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