Objective To establish the cold and hot constitution pathological models （hunger cold fatigue models

over-nutrition models） based on the ethology of animal temperature tropism to explore the correlation between the constitution and temperature tropism of mice. Methods The Kunming mice were randomized into 3 groups

i.e. normal group

hunger cold fatigue group

over-nutrition group. Food control puls swim method was used to make the hunger cold fatigue models

and high protein forage was adopted to make the over-nutrition models. An instrument with cold-hot plate was used to investigate the temperature tropism of mice

meanwhile

activities of adenosine triphosphatase （ATP） and succinate dehydrogenase （SDH）

and the total anti-oxidant capability （T-AOC） were determined to prove whether the models were made successfully. Results The hunger cold fatigue mice manifested with symptoms of "deficiency cold syndrome"

and their behavioral manifestation was significantly increased remaining rate on warm pad （40℃） （P<0.05）

suggesting an enhancement of HOT tropism. Meanwhile

the ATP

SDH

T-AOC and activity of superoxide dismutase （SOD）

oxygen consumption

and the cyclic adenosine monophosphate （cAMP）/cyclic guanosine monophosphate （cGMP） decreased significantly （P<0.05）

the activity of malondialdehyde （MDA） and the plasma cGMP increased significantly （P<0.05）

suggesting a lowering of energy metabolism and anti-oxidation ability. The manifestation of the over-nutrition mice was just opposite. Conclusion The cold-hot plate displaying method based on the animal temperature tropism shows directly and objectively the constitution difference of models with hunger cold fatigue and over-nutrition

the changes in the energy metabolism and anti-oxidation ability caused by the changes in the activities of the related enzymes maybe one of the internal mechanisms.