Effect of Buyang Huanwu Decoction Combined with Mesenchymal Stem Cells Transplantation on the Expression of bFGF mRNA in Cerebral Ischemia-reperfusion Injury Rats

ZHANG Yunke1; YANG Guanghua2

doi:10.13288/j.11-2166/r.2011.07.032

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Effect of Buyang Huanwu Decoction Combined with Mesenchymal Stem Cells Transplantation on the Expression of bFGF mRNA in Cerebral Ischemia-reperfusion Injury Rats

更新时间：2021-11-17

- Effect of Buyang Huanwu Decoction Combined with Mesenchymal Stem Cells Transplantation on the Expression of bFGF mRNA in Cerebral Ischemia-reperfusion Injury Rats
- Journal of Traditional Chinese Medicine Vol. 52, Issue 7, Pages: 589-591(2011)
- 作者机构：
  
  1. 河南中医学院第一附属医院
  2. 河南中医学院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2011.07.032
  CLC： R743;R285.5
- Published：2011
- 稿件说明：
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[1]张运克,杨广华.补阳还五汤联合间充质干细胞移植对脑缺血再灌注损伤大鼠bFGF mRNA表达的影响[J].中医杂志,2011,52(07):589-591.

ZHANG Yunke1, YANG Guanghua2. Effect of Buyang Huanwu Decoction Combined with Mesenchymal Stem Cells Transplantation on the Expression of bFGF mRNA in Cerebral Ischemia-reperfusion Injury Rats[J]. Journal of Traditional Chinese Medicine2011, 52(7): 589-591.
[1]张运克,杨广华.补阳还五汤联合间充质干细胞移植对脑缺血再灌注损伤大鼠bFGF mRNA表达的影响[J].中医杂志,2011,52(07):589-591. DOI： 10.13288/j.11-2166/r.2011.07.032.

ZHANG Yunke1, YANG Guanghua2. Effect of Buyang Huanwu Decoction Combined with Mesenchymal Stem Cells Transplantation on the Expression of bFGF mRNA in Cerebral Ischemia-reperfusion Injury Rats[J]. Journal of Traditional Chinese Medicine2011, 52(7): 589-591. DOI： 10.13288/j.11-2166/r.2011.07.032.

摘要

目的探讨补阳还五汤联合骨髓间充质干细胞（MSCs）移植对脑缺血损伤的神经保护机制。方法将48只SD大鼠随机分为假手术组、模型组、MSCs移植组（MSCs组）、补阳还五汤联合MSCs移植组（联合组）

每组12只。建立局灶性脑缺血再灌注模型

MSCs组、联合组从尾静脉注入MSCs

联合组并给予补阳还五汤

给药10天后检测碱性成纤维细胞生长因子（bFGF）基因表达情况。结果 MSCs组与联合组均能上调大鼠脑组织bFGFmRNA表达水平

与模型组和假手术组相比

差异均有统计学意义（P<0.05）

联合组表达明显高于MSCs组（P<0.05）。结论补阳还五汤联合MSCs移植能通过上调脑组织bFGFmRNA表达水平

促进内源性神经营养因子bFGF基因表达而起对脑缺血再灌注损伤起保护作用。

Abstract

Objective To study the nerve protecting function of Buyang Huanwu Decoction（Decoction of invigorating yang for recuperation）combined with bone marrow mesenchymal stem cells（MSCs）transplantation for the cerebral ischemia injury.Methods Forty-eight SD rats were randomized into sham operation group

model group

MSCs transplantation group（MSCs group）

and Buyang Huanwu Decoction combined with MSCs transplantation group（combined group）with 12 rats in each group.The models of focal cerebral ischemia-reperfusion were established.The MSCs group and combined group were treated with the tail intravenous injection of MSCs and the latter was also given Buyang Huanwu Decoction.After 10 days of treatment

the expression of basic fibroblast growth factor（bFGF）was observed.Results Both the MSCs group and the combined group could up-regulate the expression of bFGF mRNA of the brain tissue of rats.Compared with the model group and the sham operation group

there was significant difference（P<0.05）.And the combined group was the strongest one in effect（P<0.05）.Conclusion Buyang Huanwu Decoction combined with MSCs transplantation protects the brain injured by cerebral ischemia-reperfusion through up-regulating the expression of bFGF mRNA for promoting the expression of endogenous neurotrophic factor bFGF.

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