Objective To study the influencing factor of animal thermal activity based on the animal thermal activity determination system

to explore the methodology of evaluation of cold/hot nature of Chinese medicine.Methods By using the cold/hot plate differentiating instrument

the influence of different breeds

sex and adaptation time on the remaining time ratio（RTR） of ICR

BALB/c and KM mice at different temperature areas was studied.Results The tolerable temperature of ICR

BALB/c mice was between 15℃ and 40℃.Compared with before adaptation

the RTR of ICR

BALB/c and KM mice began to be depressed（P<0.05） in 6

8

and 4h in 15℃ temperature area

in 6

6

and 4h in 30℃ temperature area

and in 4

6

and 8h in 40℃ temperature area.The RTR of female and male KM mice in 15℃ to 40℃ temperature area were not significantly different（P>0.05）.Conclusion In this detective system

the tolerable temperature of ICR

BALB/c

and KM healthy mice would be 15～40℃

a relative stable memory could be obtained after 4～8h adaptation

and the perception of male and female KM mice to the same temperature might be almost the same.