Objective To evaluate the sensitization of Houttuynia Cordata Injection containing Tween-80 of different concentration and from different producer on Macaca fasciculari by intravenous injection.Methods Macaca fascicularis were randomized into 4 groups with 3 in each

i.e.normal saline group

Houttuynia Cordata Injection containing 0.25% Guangzhou chemical Tween-80 group（0.25% Guangzhou group）

Houttuynia Cordata Injection containing 0.25% Nanjing Weir Tween-80 group（0.25% Nanjing group）

and Houttuynia Cordata Injection containing 0.30% Nanjing Weir Tween-80 group（0.30% Nanjing group）.The general situations and reactions of animals were observed before intravenous infusion and within 24 hours.The reaction severity was determined according to the grading standard.Blood samples were collected before infusion and at the moment of 10min after infusion ended in order to measure the concentrations of immunoglobulin E（IgE） and histamine

and the reaction severity was also taken into consideration to evaluate comprehensively the sensitization of different injections on Macaca fascicularis.Results There were no typical anaphylactoid reactions on Macaca fascicularis induced by intravenous infusion of Houttuynia cordata injections

and the content of plasma histamine was not changed significantly compared with that before treatment.Conclusion By controlling the quality of Tween-80 and circumscribing its concentration to below 0.30%

it is possible to avoid the occurrence of anaphylactoid reactions and ensure the effect of solubilization at the same time.