Objective To investigate the effect and mechanism of acupuncture for reinforcing kidney and activating blood on learning and remembering ability of senescence-accelerated mouse P8（SAMP8） models of Alzheimer’s disease.Methods Twenty 5-month-old male SAMP8 mice were randomized into two groups

acupuncture treatment group and model control group

10 in each.Another ten 5-month-old male senescence-accelerated mouse/resistance 1（SAMR1） were prepared as normal control group.The acupuncture treatment group was treated by acupuncture with reinforcing kidney and activating blood method

the selected points were Baihui（GV20）

Xuehai（SP10）

Shenshu（BL23）

and Geshu（BL17）

and rotating the needles for reinforcing-reducing was adopted in the manipulation.The model control group and normal control group were not given special treatment.After 4 weeks

the neuroglobin（Ngb） expression in the hippocampus tissues of mice of all groups was examined by immunohistochemical method.Results The Ngb expression of the model control group was weaker than that of the normal control group（P<0.01）.Compared with the model control group

the Ngb expression of the acupuncture treatment group was significantly increased（P<0.01）.Conclusion Acupuncture invention can strengthen the Ngb expression in hippocampus tissue of SAMP8 mice.It’s possibly one of the underlying molecular mechanisms of acupuncture in improving learning and memory abilities of SAMP8 mice.