Influence of Astragalus Polysaccharide on the Expression of Toll-like Receptor 4 and Nuclear Transcription Factor-κB in Essential Hypertension Patients with Blood Stasis Syndrome

ZHANG Jingzhi 1; CHEN Liguo2; HU Xiaoqin3; JIA Huixin4

doi:10.13288/j.11-2166/r.2011.15.035

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Influence of Astragalus Polysaccharide on the Expression of Toll-like Receptor 4 and Nuclear Transcription Factor-κB in Essential Hypertension Patients with Blood Stasis Syndrome

更新时间：2021-11-17

- Influence of Astragalus Polysaccharide on the Expression of Toll-like Receptor 4 and Nuclear Transcription Factor-κB in Essential Hypertension Patients with Blood Stasis Syndrome
- Journal of Traditional Chinese Medicine Vol. 52, Issue 15, Pages: 1286-1289(2011)
- 作者机构：
  
  1. 广州医学院第二附属医院中医科
  2. 暨南大学医学院
  3. 广西中医学院
  4. 大庆油田总医院中医科
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2011.15.035
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]张竞之,陈利国,胡小勤,贾会欣.黄芪多糖对原发性高血压病血瘀证患者Toll样受体4、核转录因子-κB表达的影响[J].中医杂志,2011,52(15):1286-1289+1304.

ZHANG Jingzhi 1, CHEN Liguo2, HU Xiaoqin3, et al. Influence of Astragalus Polysaccharide on the Expression of Toll-like Receptor 4 and Nuclear Transcription Factor-κB in Essential Hypertension Patients with Blood Stasis Syndrome[J]. Journal of Traditional Chinese Medicine2011, 52(15): 1286-1289.
[1]张竞之,陈利国,胡小勤,贾会欣.黄芪多糖对原发性高血压病血瘀证患者Toll样受体4、核转录因子-κB表达的影响[J].中医杂志,2011,52(15):1286-1289+1304. DOI： 10.13288/j.11-2166/r.2011.15.035.

ZHANG Jingzhi 1, CHEN Liguo2, HU Xiaoqin3, et al. Influence of Astragalus Polysaccharide on the Expression of Toll-like Receptor 4 and Nuclear Transcription Factor-κB in Essential Hypertension Patients with Blood Stasis Syndrome[J]. Journal of Traditional Chinese Medicine2011, 52(15): 1286-1289. DOI： 10.13288/j.11-2166/r.2011.15.035.

摘要

目的以TLR-NF-κB信号转导通路的角度

探讨高血压病血瘀证的形成机制及黄芪多糖的干预作用。方法 80例高血压病患者中医辨证分为血瘀证组40例、非血瘀证组40例

另选健康志愿者（健康组）30例

收集各组受试者血清

实时PCR法测Toll样受体4（TLR4） mRNA表达。不同浓度的黄芪多糖（APS）干预脂多糖（LPS）诱导的人脐静脉内皮细胞株TLR4表达及核转录因子-κB（NF-κB）活化

PCR法测TLR4、抑制性κBα（I-κBa）mRNA的表达

免疫印迹法检测TLR4蛋白及I-κBa蛋白的表达。结果 TLR4 mRNA在血瘀证组和非血瘀证组的表达明显高于康健组（P<0.01）

血瘀证组明显高于非血瘀证组（P<0.01）;LPS诱导组TLR4 mRNA表达明显高于康健组、I-κBa mRNA明显低于健康组（P<0.01）;APS大、中、小剂量组与LPS诱导组比较

TLR4、I-κBa mRNA表达差异均有统计学意义（P<0.01）;并且APS大、中、小剂量组之间TLR4、I-κBa mRNA表达比较差异亦均有统计学意义（P<0.01）。结论高血压血瘀证患者较非血瘀证患者存在着更为严重的炎症反应和免疫紊乱

TLR-NF-κB信号途径介导的炎症反应和免疫紊乱是高血压病血瘀证形成的机制之一。

Abstract

Objective To discuss the formation mechanism of blood-stasis syndrome in essential hypertension（EH）through the TLR-NF-κB signal transduction pathway and the effect of astragalus polysaccharide.Methods Eighty patients with EH were differentiated into blood-stasis syndrome group of 40 cases and non-blood-stasis syndrome group of 40 cases

and 30 healthy people were selected from volunteers（healthy group）.The serum of each subject of all groups was collected.The real-time PCR method was adopted to measure the expression of Toll-like receptor 4（TLR4） mRNA.Different concentrations of astragalus polysaccharide（APS）were given to intervene the expression of human umbilical venous endothelial cell clone TLR4 and the activation of nuclear transcription factors-κB（NF-κB）induced by lipopolysaccharide（LPS）.The PCR method was used to measure the expression of TLR4 and inhibitory κBα（I-κBa）mRNA and the immune imprinting method was applied to detect the expression of TLR4 protein and I-κBa protein.Results The expression of TLR4 mRNA in blood-stasis group and non-blood-stasis group was significantly higher than that of healthy group（P<0.01）

and it was significantly higher in the blood-stasis group than in the non-blood-stasis group（P<0.01）.The expression of TLR4 mRNA in the LPS induction group was significantly higher and the expression of I-κBa mRNA was significantly lower than that in the healthy group（P<0.01）.In the comparison of the APS big-

medium- and small-dose groups with the LPS induction group

the expressions of TLR4 and I-κBa mRNA were both statistically different（P<0.01）.And in the comparison among the APS big-

medium- and small-dose groups

the difference of expressions of TLR4 and I-κBa mRNA was also statistically significant（P<0.01）.Conclusion Hypertension patients with blood-stasis syndrome have more serious inflammation and immune disorder than those with non-blood-stasis syndrome.The inflammation and immune disorder mediated by TLR-NF-κB signal pathway is one of the mechanisms of formation of blood-stasis syndrome of EH.

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