Influence of Yishen Tiaogan Decoction Ⅱ on the Expression of Luteinizing Hormone Receptor of Polycystic Ovarian Syndrome Model Rats

CHEN Qian; SUN Yunsong; SUN Xuan; ZHANG Bin; XU Huijun

doi:10.13288/j.11-2166/r.2011.16.033

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Influence of Yishen Tiaogan Decoction Ⅱ on the Expression of Luteinizing Hormone Receptor of Polycystic Ovarian Syndrome Model Rats

更新时间：2021-11-17

- Influence of Yishen Tiaogan Decoction Ⅱ on the Expression of Luteinizing Hormone Receptor of Polycystic Ovarian Syndrome Model Rats
- Journal of Traditional Chinese Medicine Vol. 52, Issue 16, Pages: 1409-1412(2011)
- 作者机构：
  
  青岛市海慈医院妇科
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2011.16.033
  CLC： R285.5;R711.75
- Published：2011
- 稿件说明：
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[1]陈芊,孙云松,孙选,张斌,徐慧军.益肾调肝Ⅱ号方对多囊卵巢综合征大鼠卵巢黄体生成素受体表达的影响[J].中医杂志,2011,52(16):1409-1412.

CHEN Qian, SUN Yunsong, SUN Xuan, et al. Influence of Yishen Tiaogan Decoction Ⅱ on the Expression of Luteinizing Hormone Receptor of Polycystic Ovarian Syndrome Model Rats[J]. Journal of Traditional Chinese Medicine2011, 52(16): 1409-1412.
[1]陈芊,孙云松,孙选,张斌,徐慧军.益肾调肝Ⅱ号方对多囊卵巢综合征大鼠卵巢黄体生成素受体表达的影响[J].中医杂志,2011,52(16):1409-1412. DOI： 10.13288/j.11-2166/r.2011.16.033.

CHEN Qian, SUN Yunsong, SUN Xuan, et al. Influence of Yishen Tiaogan Decoction Ⅱ on the Expression of Luteinizing Hormone Receptor of Polycystic Ovarian Syndrome Model Rats[J]. Journal of Traditional Chinese Medicine2011, 52(16): 1409-1412. DOI： 10.13288/j.11-2166/r.2011.16.033.

摘要

目的观察益肾调肝汤Ⅱ号方对多囊卵巢综合征（PCOS）模型大鼠卵泡发育、卵巢黄体生成素受体（LHR）表达的影响。方法 24日龄Wistar大鼠进行皮下埋植左炔诺孕酮结合皮下注射人绒毛膜促性腺激素造模

将造模成功60只大鼠随机分为6组

每组10只

未造模10只大鼠为正常组。分组灌服生理盐水、盐酸二甲双胍片、益肾调肝汤Ⅰ号方、益肾调肝汤Ⅱ号方低、中、高剂量15天后

观察动物卵巢重量

光镜观察卵巢卵泡的大小、黄体数;免疫组化分析LHR在卵巢中的表达情况。结果运用益肾调肝汤Ⅱ号方中、高剂量后

大鼠发育卵泡数增加

不成熟的早期发育卵泡减少

黄体数量增加

LHR表达增加。结论益肾调肝汤Ⅱ号方能明显增加大鼠卵巢重量、减少小卵泡数、增加黄体数

可明显增强LHR在卵巢中的表达

有利于卵泡的发育及排卵。

Abstract

Objective To observe the intervention effect of Yishen Tiaogan Decoction Ⅱ（Decoction for reinforcing the kidney and regulating the liver） on the follicular development and ovarian luteinizing hormone receptor（LHR） expression of model rats with polycystic ovarian syndrome（PCOS）.Methods Twenty-four-day old female Wistar rats were made PCOS models with embedding levonorgestrel stick subcutaneously in combination with injecting HCG into subcutaneous tissue.Ten rats which were not made models were put into the normal group and the 60 PCOS model rats were randomized into 6 groups

with 10 in each.After intragastric feeding with normal saline

metformin

and Yishen Tiaogan Decoction Ⅰ

low-dose

medium-dose and high-dose of Yishen Tiaogan Decoction Ⅱ respectively for 15 days

the ovary was weighted

the size of follicle and number of luteum were observed with hematoxylin-eosin（HE） staining and optical microscope

and the expression of LHR in ovary was determined with immunohistochemical analysis.Results After using the medium-dose and high-dose of Yishen Tiaogan Decoction Ⅱ

the number of follicles of models rats increased

the immature follicles decreased

the quantity of luteum increased

and the expression of LHR enhanced.Conclusion Yishen Tiaogan Decoction Ⅱ is able to significantly increase the weight of ovary of rats

reduce the number of immature follicles

increase the quantity of luteum

and enhance the LHR expression in ovary

benefiting the development of follicles and ovulation.

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