Objective To explore the clinical significance of serum anti-cyclic citrullinated peptide（anti-CCP） antibody

rheumatoid factor（RF）

glucose-6-phosphate isomerase（GPI）

and anti-keratin antibody（AKA） in diagnosing the dampheat and cold-damp Bi-syndromes of TCM in rheumatoid arthritis（RA）.Methods The clinical data and serum of 305 patients with RA were collected.The anti-CCP

RF

and GPI samples of 276 patients and the AKA samples of 253 patients were enrolled.There were 146 patients with cold-damp Bi-syndrome and 159 patients with damp-heat Bi-syndrome.The patients were assessed with disease activity scores [DAS28（4）].Level of AKA

RF

anti-CCP and GPI was determined

and difference of anti-CCP

RF

GPI and AKA between damp-heat syndrome and cold-damp syndrome as well as the correlation with DAS28（4） was analyzed.Results The levels of anti-CCP

RF

GPI

and AKA were not statistically different in the damp-heat and cold-damp Bi-syndromes of RA（P>0.05）.There was statistical difference in the disease activity between the two groups（P<0.01）.The anti-CCP

RF

and GPI were positively correlated with the number of tender joints

number of swelling joints

time of morning stiffness

DAS28（4）

and CRP respectively（P<0.01）.Conclusion The anti-CCP

RF

GPI

and AKA cannot be taken as the objective indications of classifying the damp-heat and cold-damp Bi-syndromes of RA.The anti-CCP

RF

and GPI are correlated with the disease activity to a certain degree.