Objective To explore the effect of Dodder-containing serum on the metabolism of rat osteoblasts（OB） cultured in vitro.Methods The Dodder-containing serum was prepared by serological pharmacological method

and the improved tissue block culture was adopted to culture the osteoblasts in vitro of newly born SD rats.After the culture of OB（n=8） for 24

48

and 72 hours with 5%

10%

15%

and 20% Dodder-containing serum respectively

the MTT method was used to observe the proliferation

the PNPP matrical dynamic method was applied for observing the activity of intracellular alkaline phosphatase（ALP）

and after 48h culture

the RT-PCR technique was employed to detect the COL-I mRNA expression.Results After 48h and 72h culture

the 5% and 10% Dodder-containing serum functioned significantly in improving the proliferation of OB and enhancing the activity of ALP.After 48h culture

the 10% Dodder-containing serum functioned in promoting the COL-I mRNA expression.Conclusion The 10% Dodder-containing serum can significantly promote the proliferation and differentiation of OB

indicating that it benefits osteogenesis.