Effect of Zhonglun′e Decoction on the Expression of Inflammatory Cytokines in Rats with Collagen Induced Arthritis

GAO Zili; DONG Qiumei; WANG Bin; SONG Lin; SHEN Jinghua

doi:10.13288/j.11-2166/r.2012.07.009

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Effect of Zhonglun′e Decoction on the Expression of Inflammatory Cytokines in Rats with Collagen Induced Arthritis

更新时间：2021-11-17

- Effect of Zhonglun′e Decoction on the Expression of Inflammatory Cytokines in Rats with Collagen Induced Arthritis
- Journal of Traditional Chinese Medicine Vol. 53, Issue 7, Pages: 599-602(2012)
- 作者机构：
  
  1. 内蒙古医学院
  2. 内蒙古医学院中医学院
  3. 内蒙古医学院蒙医药学院
  4. 内蒙古医学院基础学院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2012.07.009
  CLC： R29
- Published：2012
- 稿件说明：
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[1]高自立,董秋梅,王滨,松林,沈敬华.蒙药忠伦阿汤对胶原诱导性关节炎大鼠血清炎性细胞因子的影响[J].中医杂志,2012,53(07):599-602.

GAO Zili, DONG Qiumei, WANG Bin, et al. Effect of Zhonglun′e Decoction on the Expression of Inflammatory Cytokines in Rats with Collagen Induced Arthritis[J]. Journal of Traditional Chinese Medicine2012, 53(7): 599-602.
[1]高自立,董秋梅,王滨,松林,沈敬华.蒙药忠伦阿汤对胶原诱导性关节炎大鼠血清炎性细胞因子的影响[J].中医杂志,2012,53(07):599-602. DOI： 10.13288/j.11-2166/r.2012.07.009.

GAO Zili, DONG Qiumei, WANG Bin, et al. Effect of Zhonglun′e Decoction on the Expression of Inflammatory Cytokines in Rats with Collagen Induced Arthritis[J]. Journal of Traditional Chinese Medicine2012, 53(7): 599-602. DOI： 10.13288/j.11-2166/r.2012.07.009.

摘要

目的探讨蒙药忠伦阿汤对类风湿关节炎（RA）模型大鼠抗Ⅱ型胶原（CⅡ）抗体及白细胞介素1（IL-1）、白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）炎性细胞因子的影响。方法取Wistar大鼠80只

随机选取68只造模

其余12只作为空白组

以牛Ⅱ型胶原皮下注射诱导建立胶原诱导性关节炎（CIA）大鼠模型

再将造模成功的62只大鼠随机分为模型组及忠伦阿汤低、中、高剂量组和雷公藤组各12只

剩余大鼠剔除。忠伦阿汤低、中、高剂量组分别予忠伦阿汤0.35g/kg、0.7g/kg、1.5g/kg

雷公藤组予雷公藤多苷片5mg/kg

空白组和模型组给予等体积生理盐水

各组灌胃给药6周后

采用酶联免疫吸附法（ELISA）测定抗CⅡ抗体、IL-1、IL-6、TNF-α的水平。结果与空白组比较

模型组血清中抗CⅡ抗体、IL-1、IL-6、TNF-α含量均明显升高（P<0.01）

各药物组均可降低血清抗CⅡ抗体及炎性细胞因子的含量

忠伦阿汤中剂量组、高剂量组与雷公藤组在降低IL-1、IL-6、TNF-α水平方面差异无统计学意义（P>0.05）。结论蒙药忠伦阿汤可能通过调节免疫

降低血清IL-1、IL-6及TNF-α的含量

减轻炎性细胞因子对关节炎的刺激作用

发挥干预类风湿关节炎的作用。

Abstract

Objective To observe the effect of Zhonglun′e Decoction a Mongolia medicine

on the expression of anti-CⅡantibody

interleukin-1（IL-1）

interleukin-6（IL-6）and tumor necrosis factor-α（TNF-α）in rats with collagen induced arthritis （CIA）.Methods Eighty Wistar rats were randomized into control group（n=12）and model group（n=68）.CIA model was established through subcutaneous injection of typeⅡcollagen.Sixty selected models were randomized into six groups

i.e.the model group

Zhonglun′e Decoction small-dose group

Zhonglun′e Decoction middle-dose group

Zhonglun′e Decoction largedose group and tripterygium group

with 12in each.The Zhonglun′e Decoction small-dose group

middle-dose group and largedose group were given 0.35g/kg

0.7g/kg and 1.5g/kg of Zhonglun′e Decoction respectively.The tripterygium group was given 5mg/kg of tripterygium tablet.The control group and model group were given normal saline in the equal amount.The levels of anti-CⅡantibody

IL-1

IL-6and TNF-αwere detected by enzyme-linked immunosorbent assay（ELISA）after the administration through gastric feeding for 6weeks.Results Comparing with the control group

the levels of anti-CⅡantibody

IL-1

IL-6and TNF-αwas significantly increased in the model group（P<0.01）.The levels of anti-CⅡantibody and inflammatory cytokine were significantly decreased in the treatment groups.Both large-dose and middle-dose of Zhonglun′e Decoction as well as tripterygium tablet could decrease IL-1

IL-6and TNF-αwith similar effect.Conclusion Zhonglun′e Decoction could treat CIA by regulating immunity and decreasing the content of IL-1

IL-6and TNF-α.

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