Influence of Serum Containing Jiangzhi Pinggan Decoction on the Insulin Resistan ce in 3T3-L1 Adipocytes

MIAO Yuchuan1; LIU Yang1; GUO Jianhong2; HAN Dewu2; ZHAO Jiahui2; ZH AO Yuanchang2

doi:10.13288/j.11-2166/r.2012.09.029

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Influence of Serum Containing Jiangzhi Pinggan Decoction on the Insulin Resistan ce in 3T3-L1 Adipocytes

更新时间：2021-11-17

- Influence of Serum Containing Jiangzhi Pinggan Decoction on the Insulin Resistan ce in 3T3-L1 Adipocytes
- Journal of Traditional Chinese Medicine Vol. 53, Issue 9, Pages: 778-781(2012)
- 作者机构：
  
  1. 山西中医学院基础医学院
  2. 山西医科大学
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2012.09.029
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]苗宇船,刘杨,郭建红,韩德五,赵嘉慧,赵元昌.降脂平肝汤含药血清对大鼠3T3-L1脂肪细胞胰岛素抵抗的影响[J].中医杂志,2012,53(09):778-781.

MIAO Yuchuan1, LIU Yang1, GUO Jianhong2, et al. Influence of Serum Containing Jiangzhi Pinggan Decoction on the Insulin Resistan ce in 3T3-L1 Adipocytes[J]. Journal of Traditional Chinese Medicine2012, 53(9): 778-781.
[1]苗宇船,刘杨,郭建红,韩德五,赵嘉慧,赵元昌.降脂平肝汤含药血清对大鼠3T3-L1脂肪细胞胰岛素抵抗的影响[J].中医杂志,2012,53(09):778-781. DOI： 10.13288/j.11-2166/r.2012.09.029.

MIAO Yuchuan1, LIU Yang1, GUO Jianhong2, et al. Influence of Serum Containing Jiangzhi Pinggan Decoction on the Insulin Resistan ce in 3T3-L1 Adipocytes[J]. Journal of Traditional Chinese Medicine2012, 53(9): 778-781. DOI： 10.13288/j.11-2166/r.2012.09.029.

摘要

目的观察降脂平肝汤含药血清对大鼠3T3-L1脂肪细胞胰岛素受体底物1（IRS-1）mRNA和过氧化物酶增殖体激活受体（PPAR）-γmRNA表达的影响

探讨其对脂肪细胞胰岛素抵抗影响的可能机制。方法选择Wistar大鼠16只

诱导分化3T3-L1脂肪前体细胞为成熟的脂肪细胞并以肿瘤坏死因子α（TNFα）诱导建立脂肪细胞的胰岛素抵抗模型

取诱导成功的胰岛素抵抗模型细胞分为对照组（基础培养液加入20%大鼠空白血清）、模型组（基础培养液加入20%大鼠空白血清和20ng/mlTNF-α）、罗格列酮组（基础培养液加入20%大鼠含药血清和20ng/mlTNF-α）、降脂平肝汤组（基础培养液加入20%大鼠含药血清和20ng/mlTNF-α）每组4只

培养48h

观察细胞IRS-1mRNA和PPAR-γmRNA表达水平的变化。结果模型组IRS-1mRNA和PPAR-γmRNA表达明显降低

与对照组比较差异有统计学意义（P<0.05）;罗格列酮组与降脂平肝汤组IRS-1mRNA和PPAR-γmRNA表达均明显增高

与模型组比较差异有统计学意义（P<0.05）。结论降脂平肝汤可能通过增强大鼠IRS-1、PPAR-γ基因的表达起到抑制脂肪细胞胰岛素抵抗的作用。

Abstract

Objective To observe the influence of serum containin g Jiangzhi Pinggan Decoction（Decoction for lowering lipid and calming the liver） on the expressio n of insulin receptor substrate-1（IRS-1） mRNA and peroxisome proliferator-ac tivated receptor γ（PPAR-γ） mRNA in 3T3-L1 adipocytes in rats and discuss its mecha nism.Methods Sixteen Wistar rat were selected.The 3T3-L1 preadipocytes were cultured in vitro and differentia ted to adipocytes.The cell model of insulin resistance（IR） was induced by tumor necrosi s factor-α（TNF-α）.The control group was established by adding 20% o f blank serum to the culture medium.The model group was established by adding 20n g/ml of TNF-α on the basis of the control group.The rosiglitazone group and J iangzhi Pinggan group was added the corresponding bbc-containing serum respectiv ely besides the culture medium and 20ng/ml of TNF-α.The expression of IRS-1 mRN A and PPAR-γ mRNA was observed after culturing for 48 hours.R esults Comparing with the control group

the expression of IRS-1 mRNA and PPAR-γ mRNA was sign ificantly decreased in the model group（P<0.05）.The expression of IRS-1 mRN A and PPAR-γ mRNA in the rosiglitazone group was similar to that in the Jiangzhi Pin ggan Decoction group

but the differences were statistically significant between the model group and both medication groups（P<0.05）.Conclu sion Jiangzhi Pinggan Decoction could inhibit the IR of 3T3-L1 adipocytes

which maybe relevant to e nhancing the expression of IRS-1 mRNA and PPAR-γ mRNA

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