Objective To observe the effect of Jiawei Wendan Deco ction（Supplemente d Gallbladder-Warming Decoction） on calmodulin-dependent protein kinase（CaMK） s ignaling pathway in the hippocampus of depression model rats.Methods The rat models w ere established with unpredictable chronic stress and isolation-induced depress ion.Then 128 models were randomized into blank group

model group

Chinese m edication group and western medication group

with 32 in each.The Chinese medic ation group and western medication group was given 12g/（kg·d） of Jiawei Wendan Decoction and 1.8mg/（kg·d） of fluoxetine through gastric feeding respectively. Both the model group and blank group were given 2ml/（kg·d） of normal saline thro ugh gastric feeding.The administration lasted for 28 days. On the 7th

14th

21st and 28th day

the protein expression of N-methyl-D-aspartate receptor 1（NMDA-NR1）

calmodulin（CaM） and CaMKⅡ in the hippocampus of rat models were tested with Western-Blot technique.Results The re was no statistical significance in the protein expression of NMDA-NR1

CaM a nd CaMKⅡamong groups on the 7th and 14th day of administration（P>0.05）.On the 21st and 28th day

the protein expression of NMDA-NR1

CaM and CaMKⅡ increased significantly in the model group as compared with the blank group（P<0.01）. The protein expression of NMDA-NR1

CaM and CaMKⅡ decreased significantly in the t wo medication groups as compared with the model group（P<0.05 or P<0.01）.The differen ce between two medication groups was not statistically significant at the same time p eriod（P>0.05）.Conclusion Jiawei Wendan Decoction has an antidepressant effect by inhibiting the activity of CaMK signaling pathway.