Effect of Shenqi Fuzheng Injection on Proliferation and Cytotoxity of CIK Cells Originated from Umbilical Cord Blood

YAN Weiren; YU Qin; LIU Lihua; WANG Wei; YANG Longkui; FENG Li; GUAN Bo; RAN Jianying; TU Fangfang; GUO Xuping; YU Fuguo

doi:10.13288/j.11-2166/r.2012.14.024

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Effect of Shenqi Fuzheng Injection on Proliferation and Cytotoxity of CIK Cells Originated from Umbilical Cord Blood

更新时间：2021-11-17

- Effect of Shenqi Fuzheng Injection on Proliferation and Cytotoxity of CIK Cells Originated from Umbilical Cord Blood
- Journal of Traditional Chinese Medicine Vol. 53, Issue 14, Pages: 1226-1229(2012)
- 作者机构：
  
  重庆市万州区中医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2012.14.024
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]颜维仁,余琴,刘立华,王维,杨隆奎,冯莉,管波,冉建英,涂芳芳,郭绪平,喻甫国.参芪扶正注射液对脐血CIK细胞体外增殖及杀瘤活性的影响[J].中医杂志,2012,53(14):1226-1229.

YAN Weiren, YU Qin, LIU Lihua, et al. Effect of Shenqi Fuzheng Injection on Proliferation and Cytotoxity of CIK Cells Originated from Umbilical Cord Blood[J]. Journal of Traditional Chinese Medicine2012, 53(14): 1226-1229.
[1]颜维仁,余琴,刘立华,王维,杨隆奎,冯莉,管波,冉建英,涂芳芳,郭绪平,喻甫国.参芪扶正注射液对脐血CIK细胞体外增殖及杀瘤活性的影响[J].中医杂志,2012,53(14):1226-1229. DOI： 10.13288/j.11-2166/r.2012.14.024.

YAN Weiren, YU Qin, LIU Lihua, et al. Effect of Shenqi Fuzheng Injection on Proliferation and Cytotoxity of CIK Cells Originated from Umbilical Cord Blood[J]. Journal of Traditional Chinese Medicine2012, 53(14): 1226-1229. DOI： 10.13288/j.11-2166/r.2012.14.024.

摘要

目的观察参芪扶正注射液对脐血细胞因子诱导的杀伤细胞（CIK细胞）体外增殖及杀瘤活性的影响。方法用脐血单个核细胞

诱导分化CIK细胞

分为4组。细胞因子组加入γ干扰素（IFN-γ）、白细胞介素1α（IL-1α）、白细胞介素2（IL-2）、CD3单抗细胞因子;参芪扶正注射液组:加入参芪扶正注射液;细胞因子+参芪扶正注射液组:加入参芪扶正注射液﹑IFN-γ、IL-1α、IL-2、CD3单抗细胞因子;对照组只加培养液。培养12天后CIK细胞达到增殖高峰期

用流式细胞仪检测细胞表型和增殖能力

MTT法检测CIK细胞对K562细胞的杀伤作用

ELISA法检测CIK细胞分泌的细胞因子IL-2、IFN-γ、肿瘤坏死因子α（TNF-α）水平。结果对脐血CIK细胞体外增殖的影响

在培养12天时

参芪扶正注射液组与细胞因子组比较差异无统计学意义（P>0.05）

细胞因子+参芪扶正注射液组与细胞因子组相比差异有统计学意义（P<0.01）;CIK细胞对K562细胞的杀伤作用

各实验组与对照组相比差异有统计学意义（P<0.01）

参芪扶正注射液组CIK细胞在12、16天时分泌IL-2、IFN-γ、TNF-α因子水平优于对照组（P<0.01）。结论参芪扶正注射液对脐血CIK细胞既有显著的增殖和杀瘤作用

又可促进脐血CIK细胞分泌IFN-γ、IL-2和TNF-α。

Abstract

Objective To investigate the effect of Shenqi Fuzheng Injection（Ginseng and Astragalus Right-Supporting Injection） on proliferation and cytotoxity of cytokine-induced killer（CIK） cells originated from umbilical cord blood.Methods CIK cells were induced and differentiated from the cord blood mononuclear cells and then were divided into Cytokine group

Shenqi Fuzheng Injection group

Cytokine plus Shenqi Fuzheng Injection group and the control group.Cytokine group was added γ-interferon（IFN-γ）

interleukin（IL）-1α

IL-2 and CD3.Shenqi Fuzheng Injection group was added Shenqi Fuzheng Injection.Cytokine plus Shenqi Fuzheng Injection group was added Shenqi Fuzheng Injection and IFN-γ

IL-1α

IL-2 and CD3.The control group was added only cluture solution.After 12 days of cultivation

the proliferation of CIK cells reached the peak.The phenotype and proliferative capacity of CIK cells was detected with flow cytometry

the cytotoxity was detected by MTT assay

and the level of IL-2

IFN-γ and tumor necrosis factor（TNF）-α was detected by ELISA assay.Results On the 12th day of cultivation

there was no significant difference in the proliferation of CIK cells between Cytokine group and Shenqi Fuzheng Injection group（P>0.05）

but with significant difference between Cytokine group and Cytokine plus Shenqi Fuzheng Injection group（P<0.01）.The killing effect of CIK cells against K562 cells in Cytokine group

Shenqi Fuzheng Injection group and Cytokine plus Shenqi Fuzheng Injection group was better than that of the control group（P<0.01）.CIK cells in Shenqi Fuzheng Injection group could secrete IL-2

IFN-γ and TNF-α.Conclusion Shenqi Fuzheng Injection could improve the proliferation and cytotoxicity of CIK cells and promote the secretion of IFN-γ

IL-2 and TNF-α as well.

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