Effect of Three Therapies of Regulating and Supplementing the Lung and Kidney on the Cytokine Secretion of THP-1 Monocytes and Macrophages Stimulated with Cigarette Smoke Extract and Lipopolysaccharide

CHEN Yulong; WU Yaosong; LI Jiansheng; HE Lili; TIAN Yange; LI Ya

doi:10.13288/j.11-2166/r.2012.22.014

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Effect of Three Therapies of Regulating and Supplementing the Lung and Kidney on the Cytokine Secretion of THP-1 Monocytes and Macrophages Stimulated with Cigarette Smoke Extract and Lipopolysaccharide

更新时间：2021-11-17

- Effect of Three Therapies of Regulating and Supplementing the Lung and Kidney on the Cytokine Secretion of THP-1 Monocytes and Macrophages Stimulated with Cigarette Smoke Extract and Lipopolysaccharide
- Journal of Traditional Chinese Medicine Vol. 53, Issue 22, Pages: 1948-1953(2012)
- 作者机构：
  
  河南中医学院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2012.22.014
  CLC： R259
- Published：2012
- 稿件说明：
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[1]陈玉龙,吴耀松,李建生,赫丽丽,田燕歌,李亚.调补肺肾三法对香烟烟雾提取物和脂多糖刺激单核巨噬细胞THP-1分泌细胞因子的影响[J].中医杂志,2012,53(22):1948-1953.

CHEN Yulong, WU Yaosong, LI Jiansheng, et al. Effect of Three Therapies of Regulating and Supplementing the Lung and Kidney on the Cytokine Secretion of THP-1 Monocytes and Macrophages Stimulated with Cigarette Smoke Extract and Lipopolysaccharide[J]. Journal of Traditional Chinese Medicine2012, 53(22): 1948-1953.
[1]陈玉龙,吴耀松,李建生,赫丽丽,田燕歌,李亚.调补肺肾三法对香烟烟雾提取物和脂多糖刺激单核巨噬细胞THP-1分泌细胞因子的影响[J].中医杂志,2012,53(22):1948-1953. DOI： 10.13288/j.11-2166/r.2012.22.014.

CHEN Yulong, WU Yaosong, LI Jiansheng, et al. Effect of Three Therapies of Regulating and Supplementing the Lung and Kidney on the Cytokine Secretion of THP-1 Monocytes and Macrophages Stimulated with Cigarette Smoke Extract and Lipopolysaccharide[J]. Journal of Traditional Chinese Medicine2012, 53(22): 1948-1953. DOI： 10.13288/j.11-2166/r.2012.22.014.

摘要

目的探讨调补肺肾三法治疗慢性阻塞性肺疾病的分子机理。方法用香烟烟雾提取物（CSE）和脂多糖（LPS）及两者联合（LC）体外刺激单核巨噬细胞THP-1

每种刺激物下分为正常组（加入20%正常大鼠血清）、刺激物组（加入20%正常大鼠血清和5%CSE

或75pg/mlLPS

或5%CSE和75pg/mlLPS）、补肺益肾组、补肺健脾组和益气滋肾组（加20%相应方药大鼠含药血清和5%CSE

或75pg/mlLPS

或5%CSE和75pg/mlLPS）

检测各组肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-3（IL-3）、巨噬细胞-粒细胞集落刺激因子（GM-CSF）、转化生长因子-β（TGF-β）、基质金属蛋白酶-9（MMP-9）、金属蛋白酶组织抑制因子-1（TIMP-1）、超氧化物歧化酶（SOD）、CD36、磷脂酸磷酸酶2B（PPAP2B）

应用网络工具分析细胞因子的转录因子。结果与正常组比较

CSE组、LPS组和LC组分泌MMP-9增加

分泌TNF-α和PPAP2B减少;CSE组分泌TIMP-1减少

LPS组分泌TGF-β增加

LC组GM-CSF分泌增加（P<0.05或P<0.01）。3个复方对细胞分泌的各因子调节明显不同（P<0.05或P<0.01）

其中补益肺肾方在各条件下

所调节分泌的因子和涉及转录因子较为广泛。结论 CSE和LPS对THP-1分泌细胞因子影响不同

调补肺肾三法在一定程度上都对CSE和LPS刺激下分泌细胞因子有调控作用

补益肺肾方药作用最明显而广泛

补肺健脾方次之。

Abstract

Objective To analyze the molecular mechanism of three formula of regulating and supplementing the lung and kidney for chronic obstructive pulmonary disease（COPD）.Methods THP-1 monocytes and macrophages were stimulated with cigarette smoke extract（CSE）

lipopolysaccharide（LPS）or their combination（LC）in vitro.Every kinds of stimulants were divided into normal group（20%normal rat serum was added）

stimulant group（20%normal rat serum and 5%CSE

or 75pg/ml LPS

or 5%CSE and 75pg/ml LPS were added）

supplementing the lung and kidney group

supplementing the lung and spleen group

and boosting qi enriching the kidney group（20%rat serum containing relevant formula and 5%CSE

or 75 pg/ml LPS

or 5%CSE and 75pg/ml LPS were added）.Tumor necrosis factor-α （TNF-α ）

interleukin-1β（IL-1β）

interleukin 3（IL-3）

granulocyte-macrophage colony stimulating factor（GM-CSF）

transforming growth factor-β（TGF-β）

matrix metalloproteinase-9（MMP-9）

tissue inhibitor of metalloproteinase 1 （TIMP-1）

superoxide dismutase（SOD）

CD36and phosphatidic acid phosphatase 2B（PPAP2B）were detected in all groups.The transcription factor of cytokine was analyzed with network tools.Results Comparing with the normal group

the secretion of MMP-9 was increased and the secretion of TNF-α and PPAP2B was decreased in CSE group

LPS group and LC group;the secretion of TIMP-1 was decreased in CSE group

the secretion of TGF-β was increased in LPS group and the secretion of GM-CSF was increased in LC group（P<0.05or P<0.01）.The regulation to the cytokine secretion of three formula was different（P<0.05or P<0.01）.More cytokines and transcription factors could be regulated by supplementing the lung and kidney formula.Conclusion The effect of CSE and LPS on the cytokine secretion of THP-1 cells is different.All the three formula of regulating and supplementing the lung and kidney could regulate the cytokine secretion of THP-1 cells stimulated with CSE and LPS

among which the effect of supplementing the lung and kidney formula is obvious and broad

followed by supplementing the lung and spleen formula.

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Anhui University of Traditional Chinese Medicine

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