Effect of Jinfu’an Decoction on the Malignant Features of A549 Human Lung Adenocarcinoma Cells

CAO Yang1; CHEN Wenhua2; ZHOU Jingxu1; ZHOU Jianhong2; WU Yusheng1; LI Yan2

doi:10.13288/j.11-2166/r.2013.03.027

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Effect of Jinfu’an Decoction on the Malignant Features of A549 Human Lung Adenocarcinoma Cells

更新时间：2021-11-17

- Effect of Jinfu’an Decoction on the Malignant Features of A549 Human Lung Adenocarcinoma Cells
- Journal of Traditional Chinese Medicine Vol. 54, Issue 3, Pages: 241-244(2013)
- 作者机构：
  
  1. 广州中医药大学第一附属医院
  2. 广州中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2013.03.027
  CLC： R734.2
- Published：2013
- 稿件说明：
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[1]曹洋,陈文华,周京旭,周健洪,吴玉生,李雁.金福安汤对人肺腺癌A549细胞生物学行为的影响[J].中医杂志,2013,54(03):241-244.

CAO Yang1, CHEN Wenhua2, ZHOU Jingxu1, et al. Effect of Jinfu’an Decoction on the Malignant Features of A549 Human Lung Adenocarcinoma Cells[J]. Journal of Traditional Chinese Medicine2013, 54(3): 241-244.
[1]曹洋,陈文华,周京旭,周健洪,吴玉生,李雁.金福安汤对人肺腺癌A549细胞生物学行为的影响[J].中医杂志,2013,54(03):241-244. DOI： 10.13288/j.11-2166/r.2013.03.027.

CAO Yang1, CHEN Wenhua2, ZHOU Jingxu1, et al. Effect of Jinfu’an Decoction on the Malignant Features of A549 Human Lung Adenocarcinoma Cells[J]. Journal of Traditional Chinese Medicine2013, 54(3): 241-244. DOI： 10.13288/j.11-2166/r.2013.03.027.

摘要

目的观察金福安汤对人肺腺癌A549细胞增殖、p120ctn磷酸化、细胞黏附及迁移的影响并探讨其治疗肺癌的作用机制。方法培养A549细胞

分为空白对照组

金福安汤低、中、高剂量组

环磷酰胺组

分别给予含空白血清和含药血清培养液干预。检测各组A549细胞OD值并计算抑瘤率;刺激A549细胞p120ctn磷酸化后

检测各组A549细胞p120ctn灰度值;并观察各组p120ctn磷酸化的A549细胞黏附和迁移。结果金福安汤高、中、低剂量组A549细胞OD值与空白对照组比较差异有统计学意义

且呈剂量相关性（P<0.05或P<0.01）;金福安汤中剂量组A549细胞p120ctn磷酸化程度较空白对照组显著降低（P<0.05）;金福安汤中、高剂量组A549细胞的黏附能力较空白对照组高（P<0.05）

金福安汤各剂量组A549细胞的迁移能力较空白对照组下降（P<0.05）。结论金福安汤具有抑制A549细胞增殖及其p120ctn磷酸化、促进细胞黏附和抑制迁移的作用。

Abstract

Objective To observe the inhibition of Jinfu’an Decoction on A549human lung adenocarcinoma cells（A549 cells）and explore its mechanism for lung cancer.Methods A549cells were cultured and randomized into blank control group

Jinfu’an Decoction small-dose

middle-dose and large-dose groups and cyclophosphamide group.The blank serum-containing or drug serum-containing culture medium was given to each group respectively.The inhibition of Jinfu’an Decoction on A549cells was detected with MTT assay.A549cells were stimulated to make p120ctn tyrosine phosphorylation and p120ctn was detected with immunoprecipitation and western blot.The adhesion and migration of A549cells were observed.Results Jinfu’an Decoction had an obvious dose-dependent inhibitory effect on A549cells.Comparing with the blank control group

p120ctn tyrosine phosphorylation of A549cells was significantly decreased in the Jinfu’an Decoction middle-dose group（P<0.05）.Comparing with the blank control group

the adhesion ability of A549cells was significantly increased in the Jinfu’an Decoction middle-dose and large-dose groups（P<0.05）.Comparing with the blank control group

the migration ability of A549cells in the Jinfu’an Decoction groups was significantly decreased（P<0.05）.Conlcusion Jinfu’an Decoction can inhibit the proliferation of A549cells and p120ctn tyrosine phosphorylation

decrease the malignancy of A549cells and cure lung cancer.

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