Effects of Fengshining Capsule on the Ultrastructure of Synovial Cells and the Expressions of Apoptosis-Related Genes in Arthritic Rats Induced by Collagen

MA Yanmiao1; WANG Yonghui2; LI Yanyan2; GAO Li2; ZHOU Ran2

doi:10.13288/j.11-2166/r.2013.04.029

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Effects of Fengshining Capsule on the Ultrastructure of Synovial Cells and the Expressions of Apoptosis-Related Genes in Arthritic Rats Induced by Collagen

更新时间：2021-11-17

- Effects of Fengshining Capsule on the Ultrastructure of Synovial Cells and the Expressions of Apoptosis-Related Genes in Arthritic Rats Induced by Collagen
- Journal of Traditional Chinese Medicine Vol. 54, Issue 4, Pages: 326-328(2013)
- 作者机构：
  
  1. 湖北中医药大学研究生处
  2. 山西中医学院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2013.04.029
  CLC： R285.5
- Published：2013
- 稿件说明：
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[1]马艳苗,王永辉,李艳彦,高丽,周然.风湿宁胶囊对胶原诱导性关节炎大鼠滑膜细胞超微结构及细胞凋亡相关基因表达的影响[J].中医杂志,2013,54(04):326-328+335.

MA Yanmiao1, WANG Yonghui2, LI Yanyan2, et al. Effects of Fengshining Capsule on the Ultrastructure of Synovial Cells and the Expressions of Apoptosis-Related Genes in Arthritic Rats Induced by Collagen[J]. Journal of Traditional Chinese Medicine2013, 54(4): 326-328.
[1]马艳苗,王永辉,李艳彦,高丽,周然.风湿宁胶囊对胶原诱导性关节炎大鼠滑膜细胞超微结构及细胞凋亡相关基因表达的影响[J].中医杂志,2013,54(04):326-328+335. DOI： 10.13288/j.11-2166/r.2013.04.029.

MA Yanmiao1, WANG Yonghui2, LI Yanyan2, et al. Effects of Fengshining Capsule on the Ultrastructure of Synovial Cells and the Expressions of Apoptosis-Related Genes in Arthritic Rats Induced by Collagen[J]. Journal of Traditional Chinese Medicine2013, 54(4): 326-328. DOI： 10.13288/j.11-2166/r.2013.04.029.

摘要

目的探讨风湿宁胶囊治疗类风湿关节炎（RA）的作用机制。方法 60只SD大鼠随机分为正常对照组、模型组、雷公藤多苷片组、风湿宁低剂量组、风湿宁中剂量组、风湿宁高剂量组各10只

除正常对照组外其余各组建立胶原诱导性关节炎（CIA）大鼠模型

雷公藤多苷片组给予雷公藤多苷片0.03g/（kg.d）

风湿宁低、中、高剂量组分别给予风湿宁胶囊0.33、0.66、1.32g/（kg.d）

正常对照组和模型组给予蒸馏水

各组按20ml/kg体重灌胃

每天1次

连续4周。透射电镜观察大鼠滑膜细胞超微结构

RT-PCR检测凋亡相关基因caspase 3mRNA、caspase 9mRNA的表达水平。结果电镜下风湿宁低、中、高剂量组大鼠的滑膜细胞超微结构均较模型组有不同程度的改善

尤以风湿宁中剂量组效果明显。雷公藤多苷片组及风湿宁低、中剂量组caspase 3mRNA的表达较模型组上调（P<0.05或P<0.01）;风湿宁中、高剂量组及雷公藤多苷片组caspase 9mRNA表达较模型组上调（P<0.05或P<0.01）。结论风湿宁胶囊治疗RA机制可能与改善大鼠滑膜细胞的超微结构、上调凋亡相关基因caspase 3mRNA、caspase 9mRNA有关。

Abstract

Objective To research the mechanism of Fengshining Capsule（FSN） for rheumatoid arthritis（RA）.Methods Sixty SD rats were randomized into the normal control group

model group

Tripterygium glycosides tablet（TGT） group

low-dose FSN group

middle-dose FSN group and high-dose FSN group

with 10 in each.The rat models with collagen-induced arthritis（CIA） were established in the five groups except the normal control group.The TGT group was given 0.03 g/（kg·d） TGT.The low-dose

middle-dose and high-dose FSN groups were given 0.33 g/（kg·d）

0.66 g/（kg·d） and 1.32 g/（kg·d） FSN capsule respectively.The distilled water was given to the normal control group and model group.Each group was intragastrically administrated with 20ml/kg of corresponding medicine

once daily for 4 weeks.The ultrastructure of synovial cells was observed with transmission electron microscopy and the expressions of caspase-3 and caspase-9 mRNA were detected with reverse transcription-polymerase chain reaction（RT-PCR） method.Results Comparing with the model group

the ultrastructure of synovial cells was improved in the FSN groups

especially in the middle-dose FSN group.Comparing with the model group

the expression of caspase-3 mRNA was significantly increased in the TGT group

low-dose FSN group and middle-dose FSN group（P<0.05 or P<0.01）.Comparing with the model group

the expression of caspase-9 mRNA was significantly increased in the TGT group

high-dose FSN group and middle-dose FSN group（P<0.05 or P<0.01）.Conclusion The mechanism of FSN capsule for RA may be relevant to improving the ultrastructure of synovial cells and increasing the apoptosis-related genes caspase-3 mRNA and caspase-9 mRNA.

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