Effect of Huazhuo Jiedu Formula on DGAT2 mRNA Expression in Liver Tissue of Lipid Disorders Rats with Insulin Resistance

ZHOU Xiang1; ZHANG Qinghua2; YAN Dongxue3; JIE Yanghua1; SHI Cuijuan1; WU Shentao2

doi:10.13288/j.11-2166/r.2013.05.025

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Effect of Huazhuo Jiedu Formula on DGAT2 mRNA Expression in Liver Tissue of Lipid Disorders Rats with Insulin Resistance

更新时间：2021-11-17

- Effect of Huazhuo Jiedu Formula on DGAT2 mRNA Expression in Liver Tissue of Lipid Disorders Rats with Insulin Resistance
- Journal of Traditional Chinese Medicine Vol. 54, Issue 5, Pages: 412-414(2013)
- 作者机构：
  
  1. 天津中医药大学研究生院
  2. 天津中医药大学第一附属医院
  3. 天津市南开医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2013.05.025
  CLC： R285.5
- Published：2013
- 稿件说明：
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[1]周祥,章清华,闫冬雪,节阳华,史翠娟,吴深涛.化浊解毒方对脂代谢异常伴胰岛素抵抗大鼠肝脏组织DGAT2 mRNA表达的影响[J].中医杂志,2013,54(05):412-414.

ZHOU Xiang1, ZHANG Qinghua2, YAN Dongxue3, et al. Effect of Huazhuo Jiedu Formula on DGAT2 mRNA Expression in Liver Tissue of Lipid Disorders Rats with Insulin Resistance[J]. Journal of Traditional Chinese Medicine2013, 54(5): 412-414.
[1]周祥,章清华,闫冬雪,节阳华,史翠娟,吴深涛.化浊解毒方对脂代谢异常伴胰岛素抵抗大鼠肝脏组织DGAT2 mRNA表达的影响[J].中医杂志,2013,54(05):412-414. DOI： 10.13288/j.11-2166/r.2013.05.025.

ZHOU Xiang1, ZHANG Qinghua2, YAN Dongxue3, et al. Effect of Huazhuo Jiedu Formula on DGAT2 mRNA Expression in Liver Tissue of Lipid Disorders Rats with Insulin Resistance[J]. Journal of Traditional Chinese Medicine2013, 54(5): 412-414. DOI： 10.13288/j.11-2166/r.2013.05.025.

摘要

目的探讨化浊解毒方改善脂代谢异常伴胰岛素抵抗（IR）的作用机制。方法 30只SD大鼠随机选取10只为空白对照组

其余大鼠采用一次性尾静脉注射小剂量链脲佐菌素加高脂饲料诱导脂代谢异常伴IR模型

将造模成功的16只大鼠随机分为模型组、化浊解毒方组各8只;化浊解毒方组给予化浊解毒颗粒3g生药/（kg·d）

空白对照组与模型组给予等量生理盐水

各组均连续灌胃8周。8周后检测各组大鼠空腹血糖（FBG）、血中胰岛素（FINS）、甘油三酯（TG）、总胆固醇（TC）、游离脂肪酸（FFA）

采用实时定量PCR检测模型组和化浊解毒方组大鼠肝脏二酰基甘油酰基转移酶2（DGAT2）mR-NA表达。结果与空白对照组比较

模型组FBG、FINS、TG、TC、FFA均明显升高（P<0.01）;与模型组比较

化浊解毒方组FBG、FINS、TG、TC、FFA均显著下降（P<0.05或P<0.01）;化浊解毒方组DGAT2mRNA表达较模型组显著降低（P<0.01）。结论化浊解毒方可抑制脂代谢异常伴IR大鼠肝脏DGAT2 mRNA表达

可能是其改善脂代谢异常伴IR的机制之一。

Abstract

Objective To observe the mechanism of Huazhuo Jiedu Formula （turbidity-transforming toxin-resolving formula

HJF） for lipid disorders with insulin resistance （IR）.Methods Among 30 SD rats

10 rats were selected as the blank control group and other rats were modeled with low-dose streptozotocin （STZ） and high fat diet. Sixteen successfully modeling rats were randomized into model group and HJF group

with 8 in each. The HJF group was given 3g crude drug/（kg·d） of HJF granules. Normal saline was given to the blank control group and model group. The intragastric administration lasted for 8 weeks. Fasting blood glucose （FBG）

fasting serum insulin （FINS）

triglyceride （TG）

total cholesterol （TC） and free fatty acid （FFA） were detected 8 weeks later. The expression of diacylglycerol acyltransferase 2 （DGAT2） mRNA was examined by real time polymerase chain reaction （RT-PCR）.Results Comparing with the blank control group

FBG

FINS

TC and FFA were significantly increased in the model group （P<0.01）. Comparing with the model group

FBG

FINS

TC and FFA were significantly decreased in the HJF group （P<0.01 or P<0.05）. Comparing with the model group

DGAT2 mRNA expression was significantly decreased in the HJF group （P<0.01）.Conclusion HJF can inhibit DGAT2mRNA expression in liver tissue of lipid disorders rats with IR

which may be part mechanism for ameliorating IR and lipid disorders.

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