Objective To evaluate the short and long term effects of three methods of regulating and supplementing the lung and kidney on the right ventricular remodeling （RVR） in rats with chronic obstructive pulmonary disease （COPD）.Methods Totally 120 rats were randomized into control group

model group

lung-supplementing spleen-fortifying group

lung-supplementing kidney-boosting group

qi-boosting kidney-enriching group and aminophylline group

with 20 in each. The COPD models were established with cigarette smoke exposure and bacterial infection in the five groups except the control group. After 8 weeks of modeling

each group was intragastrically administered with the formulas of lung-supplementing spleen-fortifying

lung-supplementing kidney-boosting

qi-boosting kidney-enriching and aminophylline respectively from the 9th week to the 20th week. The rats were sacrificed and the myocardial tissue samples were obtained after 20 or 32 weeks of treatment. The ultrastructure of myocardial tissue was observed. The right ventricular hypertrophy index （RVHI） was calculated and the myocardial tissue factor expression was observed.Results Comparing with the model group

the RVHI was significantly decreased in other groups after 20 or 32 weeks of treatment （P<0.01）. Comparing with the aminophylline group

the RVHI was significantly decreased in the lung supplementing spleen fortifying group

lung supplementing kidney boosting group and qi boosting kidney enriching group （P<0.05 or P<0.01）. The sarcomere length in the model group was shorter than that in the control group after 20 or 32 weeks of treatment （P<0.05）. The sarcomere length in the lung supplementing spleen fortifying group was longer than that in the model group after 20 or 32 weeks of treatment （P<0.01）. The myocardial tissue factor expression in the model group was significantly higher than those in other groups after 20 or 32 weeks of treatment （P<0.01）. Comparing with the aminophylline group

the endothelin-1 （ET-1） expression was significantly decreased in the lung supplementing spleen fortifying group

lung supplementing kidney boosting group and qi boosting kidney enriching group （P<0.01）.Conclusion Three methods of regulating and supplementing the lung and kidney can improve the right ventricular remodeling with a obvious long term effect

especially the lung supplementing spleen fortifying formula and lung supplementing kidney boosting formula. The mechanism may be relevant to regulating cytokine.