Effect of Kangxianling on Phenotypic Transformation of Bone Marrow-Derived Fibroblast

WANG Dong1; ZHANG Changming2; WANG Yunman3; HE Liqun1

doi:10.13288/j.11-2166/r.2013.05.027

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Effect of Kangxianling on Phenotypic Transformation of Bone Marrow-Derived Fibroblast

更新时间：2021-11-17

- Effect of Kangxianling on Phenotypic Transformation of Bone Marrow-Derived Fibroblast
- Journal of Traditional Chinese Medicine Vol. 54, Issue 5, Pages: 420-423(2013)
- 作者机构：
  
  1. 上海中医药大学附属曙光医院
  2. 上海中医药大学附属市中医院
  3. 上海中医药大学附属普陀医院
  4. 上海市中医临床重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2013.05.027
  CLC： R285.5
- Published：2013
- 稿件说明：
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[1]王东,张长明,王云满,何立群.抗纤灵方药物血清对骨髓来源成纤维细胞表型转化的影响[J].中医杂志,2013,54(05):420-423.

WANG Dong1, ZHANG Changming2, WANG Yunman3, et al. Effect of Kangxianling on Phenotypic Transformation of Bone Marrow-Derived Fibroblast[J]. Journal of Traditional Chinese Medicine2013, 54(5): 420-423.
[1]王东,张长明,王云满,何立群.抗纤灵方药物血清对骨髓来源成纤维细胞表型转化的影响[J].中医杂志,2013,54(05):420-423. DOI： 10.13288/j.11-2166/r.2013.05.027.

WANG Dong1, ZHANG Changming2, WANG Yunman3, et al. Effect of Kangxianling on Phenotypic Transformation of Bone Marrow-Derived Fibroblast[J]. Journal of Traditional Chinese Medicine2013, 54(5): 420-423. DOI： 10.13288/j.11-2166/r.2013.05.027.

摘要

目的探讨抗纤灵方对肾间质纤维化（RIF）的作用机制。方法将抗纤灵方煎至含生药3.2g/ml、福辛普利0.33mg/ml给大鼠灌胃

正常组给予等量蒸馏水灌胃

分别制备抗纤灵方血清、福辛普利血清和正常血清。用DMEM培养基稀释血清

分为正常血清组、福辛普利组、抗纤灵组、转化生长因子-β1（TGF-β1）组、TGF-β1+福辛普利组和TGF-β1+抗纤灵组。体外培养大鼠肾组织骨髓来源的成纤维细胞（FBS）

采用酶联免疫吸附法和双重荧光定量法检测骨髓来源的FBS表型转化标记物α-平滑肌肌动蛋白（α-SMA）及Ⅰ型胶原（CollagenⅠ）及其mRNA表达。结果 TGF-β1组α-SMA、CollagenⅠ及α-SMAmRNA、CollagenⅠmRNA表达水平较正常血清组明显增强（P<0.05或P<0.01）

TGF-β1+福辛普利组和TGF-β1+抗纤灵组α-SMA、CollagenⅠ及α-SMAmRNA、CollagenⅠmRNA表达水平均低于TGF-β1组（P<0.05或P<0.01）。结论抗纤灵方药物血清可以通过抑制骨髓来源的FBS的表型转化拮抗RIF。

Abstract

Objective To study the effect and mechanism of Kangxianling on renal interstitial fibrosis （RIF）.Methods Kangxianling serum

fosinopril serum and normal serum was prepared respectively with intragastric administration of 3.2g/ml Kangxianling

3.3mg/ml fosinopril and distilled water to rats. The serum was diluted with a DMEM medium and divided into the normal group

fosinopril group

Kangxianling group

transforming growth factor-β1 （TGF-β1） group

TGF-β1+fosinopril group and TGF-β1+Kangxianling group. The bone marrow-derived fetal bovine serum （FBS） was studied. The expressions of α-smooth muscle actin （α-SMA） and Collagen I were examined by enzyme-linked immunosorbent assay （ELISA） and real time polymerase chain reaction （RT-PCR）.Results Comparing with the normal group

the expressions of α-SMA

Collagen I

α-SMA mRNA and Collagen I mRNA were significantly improved in the TGF-β1 group （P<0.05 or P<0.01）. Comparing with the TGF-β1 group

the expressions of α-SMA

Collagen I

α-SMA mRNA and Collagen I mRNA were significantly decreased in the TGF-β1+fosinopril group and TGF-β1+Kangxianling group （P<0.05 or P<0.01）.Conclusion fKangxianling can antagonize RIF by inhibiting phenotypic transformation of bone marrow-derived FBS.

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