Effect of Curcumin on Inflammatory Factor in Acute Pulmonary Embolism Rats

WANG Lingcong; ZHANG Wei; XIA Guolian; JIANG Ronglin

doi:10.13288/j.11-2166/r.2013.06.023

您当前的位置：

首页 >

文章列表页 >

Effect of Curcumin on Inflammatory Factor in Acute Pulmonary Embolism Rats

更新时间：2021-11-17

- Effect of Curcumin on Inflammatory Factor in Acute Pulmonary Embolism Rats
- Journal of Traditional Chinese Medicine Vol. 54, Issue 6, Pages: 506-508(2013)
- 作者机构：
  
  浙江中医药大学附属第一医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2013.06.023
  CLC： R285.5
- Published：2013
- 稿件说明：
移动端阅览
[1]王灵聪,张微,夏国莲,江荣林.姜黄素对急性肺栓塞大鼠炎症因子的影响[J].中医杂志,2013,54(06):506-508+515.

WANG Lingcong, ZHANG Wei, XIA Guolian, et al. Effect of Curcumin on Inflammatory Factor in Acute Pulmonary Embolism Rats[J]. Journal of Traditional Chinese Medicine2013, 54(6): 506-508.
[1]王灵聪,张微,夏国莲,江荣林.姜黄素对急性肺栓塞大鼠炎症因子的影响[J].中医杂志,2013,54(06):506-508+515. DOI： 10.13288/j.11-2166/r.2013.06.023.

WANG Lingcong, ZHANG Wei, XIA Guolian, et al. Effect of Curcumin on Inflammatory Factor in Acute Pulmonary Embolism Rats[J]. Journal of Traditional Chinese Medicine2013, 54(6): 506-508. DOI： 10.13288/j.11-2166/r.2013.06.023.

摘要

目的探讨姜黄素（CUR）对急性肺栓塞（APE）大鼠肺组织损伤的作用机制。方法 96只SD大鼠随机分为正常组

假手术组

模型组

CUR低、中、高剂量组

每组16只

采用自体血栓法建立APE大鼠模型。CUR低、中、高剂量组分别给予CUR50、100、200mg/kg灌胃

正常组、假手术组和模型组给予等体积生理盐水。造模前24h和造模前40min各灌胃1次

造模后每日灌胃1次。各组在造模后4h、72h分别处死动物

检测血清白介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）水平

肺组织HE染色观察病理改变

测定肺组织不规则趋化因子（CX3CL1）及受体（CX3CR1）阳性细胞数。结果造模后4h、72h

正常组、假手术组、CUR低剂量组、CUR中剂量组IL-8显著低于模型组（P<0.05或P<0.01）。造模后72h

正常组、假手术组、CUR高剂量组TNF-α显著低于模型组（P<0.05）

正常组

假手术组

CUR低、中、高剂量组IL-1β均显著低于模型组（P<0.05）。造模后72hCUR可明显减轻肺组织充血程度。造模后4h

正常组、假手术组、CUR高剂量组的CX3CL1阳性细胞计数均显著小于模型组（P<0.01）

正常组

假手术组

CUR中、高剂量组的CX3CR1阳性细胞计数显著小于模型组（P<0.01）。造模后72h

正常组

假手术组

CUR低、中、高剂量组的CX3CL1阳性细胞计数均显著小于模型组（P<0.05或P<0.01）

正常组、假手术组的CX3CR1阳性细胞计数显著小于模型组（P<0.01）。结论 CUR可抑制APE大鼠TNF-α、IL-1、IL-8、CX3CL1及CX3CR1的表达水平

这可能是其减轻APE肺组织损伤的机制之一。

Abstract

Objective To observe the mechanism of curcumin （C UR） for lung tissue injury in acute pulmonary embolism （A PE） rats.Methods Totally 96SD rats were randomized into the normal group

sham group

model group

low-dose CUR group

middle-dose CUR group and high-dose CUR group

with 16in each.The APE models were established by injecting self-thrombus.The low-dose CUR group

middle-dose CUR group and high-dose CUR group was given 50mg /kg

100mg /kg and 200mg /kg of CUR respectively.The normal group

sham group and model group was given the same volume of saline.The rats were given intragastric administration 40minutes and 24hours before modeling and once daily after modeling.The rats were sacrificed on the 4hand 72hafter modeling.The serum levels of interleukin-8 （IL-8）

tumor necrosis factor-α （TNF-α） and interleukin-1 β （IL-1 β） were measured with enzyme-linked immunosorbent assay （E LISA）.The pathological changes of lung tissue were observed with HE staining.The Chemokine （C-X3-C motif） ligand 1 （C X3CL1） and CX3Cchemokine recep-tor 1 （C X3CR1） levels in lung tissue were measured with immunohischemical method.Results The IL-8levels in the normal group

sham group

low-dose CUR group and middle-dose CUR group were significantly lower than those in the model group on the 4hand 72hafter modeling （P<0.05or P<0.01）.The TNF-αlevels in the normal group

sham group and high-dose CUR group were significantly lower than that in the model group on the 72hafter modeling （P<0.05）.The IL-1 β levels in the normal group

sham group

low-dose CUR group

middle-dose CUR group and high-dose CUR group were significantly lower than that in the model group on the 72hafter modeling （P<0.05）.On the 72hafter modeling

the hyperemia of the lung tis-sues was apparently reversal after applying CUR.The CX3CL1-postive cell counts in the normal group

sham group and high-dose CUR group were significantly lower than those in the model group on the 4hafter modeling （P<0.01）.The CX3CR1-postive cell counts in the normal group

sham group

middle-dose CUR group and high-dose CUR group were significantly low-er than those in the model group on the 4hafter modeling （P<0.01）.The CX3CL1-postive cell counts in the normal group

sham group

low-dose CUR group

middle-dose CUR group and high-dose CUR group were significantly lower than those in the model group on the 72hafter modeling （P<0.05or P<0.01）.The CX3CR1-postive cell counts in the normal group and sham group were significantly lower than those in the model group on the 72hafter modeling （P<0.01）.Conclusion The in-flammatory response appears in APE rats.CUR can inhibit the levels of IL-8

TNF-α

IL-1 β

CX3CL1and CX3CR1in APE rats

which may be relevant to relieving lung tissue injury.

关键词

Keywords

references

查看原文

Views

168

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Yigan Fupi Prescription （抑肝扶脾方） on the AKT/mTOR Pathway in the Colon Tissue of Diarrhea-Type Irritable Bowel Syndrome Model Rats with Liver Stagnation and Spleen Deficiency Pattern

Effect of Acupuncture Combined with Bloodletting and Cupping on the Expression of Coagulation-Complement-Mast Cell Activation Axis-Related Factors in Patients with Chronic Spontaneous Urticaria：Randomize-controlled Study

Comparative Study on the Pharmacodynamics of Xiangzhu Fanggan Formula （香术防感方） by Sniffing and Nasal Drops for Prevention of Influenza A H1N1 Virus Infection in Mice

Effect of Qingyu Hejiang Decoction（清郁和降汤）on LPS/TLR4/NFκB Pathway and Esophageal Mucosal Barrier in Reflux Esophagitis Model Rats

Comparing the Mechanism of Action of Huangqi Guizhi Wuwu Decoction （黄芪桂枝五物汤） and Si Shen Decoction （四神煎） for Treatment of Rheumatoid Arthritis based on Network Pharmacology

Related Author

JIU Suting

LI Huiying

SUN Yueting

CUI Songxiang

XU Xintian

ZHENG Hao

LU Weimin

DU Yuzhu

Related Institution

Pukou District Hospital of Traditional Chinese Medicine

Qujing Hospital of Traditional Chinese Medicine

Affiliated Hospital of Nanjing University of Chinese Medicine

The Third Hospital of Hebei Medical University

The Second Hospital of Hebei Medical University

Address：No.16, Nanxiao street, Dongzhimen, Dongcheng District, Beijing Postal code：100700
Tel：010-64035632 Email：jtcmcn@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010102007558号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰