Objective To observe the effects and mechanism of Qinjiao（Radix Gentianae macrophyllae）in different combinations on the serum levels of tumor necrosis factor-α（TNF-α）and interleukin-1β（IL-1β）in wind-cold-damp or wind-dampheat arthralgia model rats.Methods Totally 128Wistar rats were randomized into two major groups

i.e.the wind-cold-damp arthralgia group and wind-damp-heat arthralgia group

and then each group was further divided into the blank group

model group

Tripterygium group

single Qinjiao group

Qinjiao-Sangjisheng（Herba Taxilli）group

Qinjiao-Fangji（Radix Stephaniae Tetrandrae）group

Qinjiao-Weilingxian（Radix et Rhizoma Clematidis）group and Qinjiao-Qianghuo（Rhizoma et Radix Notopterygii）group

with 8in each.Wind-cold-damp arthralgia or wind-damp-heat arthralgia models were induced in all groups except for the blank groups by adjuvant and being exposed in wind-cold-heat or wind-damp-heat environment

respectively.From the 1st day of modeling

each administration group was gavaged with 10ml/kg corresponding drug solution and the blank and model groups were given equivalent normal saline once daily for 25consecutive days.The serum levels of TNF-αand IL-1β were detected.Results The serum levels of TNF-αand IL-1βin the wind-cold-damp arthralgia group and wind-damp-heat arthralgia group were significantly higher than those in the blank group（P<0.01）.The serum levels of TNF-αand IL-1βin all administration groups were significantly decreased in varying degrees（P<0.05or P<0.01）

especially in the Qinjiao-Qianghuo group

Qinjiao-Weilingxian group and Qinjiao-Sangjisheng group of the wind-cold-damp arthralgia models（P<0.01）.The TNF-αlevels in the Qinjiao-Qianghuo group

Qinjiao-Weilingxian group and Qinjiao-Sangjisheng group were significantly lower than that in the Qinjiao-Fangji group（P<0.05or P<0.01）.The serum levels of TNF-αand IL-1βwere significantly decreased in the Qinjiao-Fangji group

Qinjiao-Sangjisheng group and Qinjiao-Qianghuo group of wind-damp-heat arthralgia models（P<0.01）.The IL-1βlevel in the Qinjiao-Fangji group was significantly lower than that in the Qinjiao-Weilingxian group（P<0.05）.Conclusion The combinations of Qinjiao-Qianghuo

Qinjiao-Weilingxian and Qinjiao-Sangjisheng can obviously decrease the serum levels of TNF-αand IL-1βin the wind-cold-damp arthralgia model rats and the combination of Qinjiao-Fangji can obviously decrease the serum levels of TNF-αand IL-1βin the wind-damp-heat arthralgia model rats.