Objective To research the differentially expressed gene and promoter methylation status of coronary heart disease（CHD）with blood stasis pattern.Methods Fourteen family-related CHD patients with blood stasis pattern were selected as observation subjects and 7healthy persons were chosen as control.The differentially expressed genes of CHD with blood stasis pattern were detected by microarray.Another 16CHD patients with blood stasis pattern and 7healthy persons were chosen and 2differentially expressed genes were selected to conduct methylation-specific PCR（MS-PCR）of promoter region.The differentially expressed gene and promoter methylation status of CHD with blood stasis pattern was analyzed.Results A total of 26differentially expressed genes were obtained after screening the microarray results which fold change（FC）value>3or FC value<1/3and Call=P.The KLF5gene and LRP12gene were selected to conduct the MS-PCR.There was no significant difference between the CHD patients with blood stasis pattern and healthy persons in the promoter methylation rate（P> 0.05）.Conclusion There is no significant difference between the CHD patients with blood stasis pattern and healthy persons in the promoter methylation status of differentially expressed genes KLF5and LRP12.