Objective To observe the effect of different Chinese medical therapies on the expression and proliferation of Galectin-3（GAL-3） in human hepatoma cells.Methods Seventy-two male Wistar rats were randomized into the normal group

model group

Tegafur group（8.33 mg/100 g Tegafur）

Heat-Clearing Toxin-Resolving Formula group（1.5 g/100 g HCTRF）

Blood-Quickening Stasis-Transforming Formula group（1.27 g/100 g BQSTF） and Spleen-Fortifying Qi-Regulating Formula group（1.75 g/100 g SFQRF）

with 12 in each.Diethylnitrosamine（DEN） was used to induce the hepatic carcinoma rat models in each group except the normal group.The rats in the administration groups were gavaged 12 times daily for six weeks.The expression of GAL-3 in the rat hepatocellular carcinoma（HCC） tissues（liver tissues in the normal group） was detected.Two siRNA targets were designed according to human GAL-3 gene coding region and the recombinant plasmid transfection human hepatoma cell line SMMC-7721 was constructed.The gene expression was detected by real-time quantitative polymerase chain reaction（PCR） and the cell proliferation was detected by 3-（4

5-dimethylthiazol-2-yl）-2

5-diphenyltetrazolium bromide（MTT） assay 24 h

72 h

and 144 h after transfection.Results The expression of GAL-3 gene in the model group was significantly increased.SFQRF could down-regulate the expression of GAL-3 gene

the expression of GAL-3 mRNA was significantly decreased 72 h after transfection and the proliferation of hepatoma cells was significantly inhibited 144 h after transfection（P<0.01）.Conclusion The GAL-3 gene is highly expressed in HCC tissues and SFQRF can significantly down-regulate its expression.