Effect of Tanshinone Ⅱ<sub>A</sub> on the Expression of Endothelial Nitric Oxide Synthase and Phosphoinositide 3-Kinase in Human Umbilical Vein Endothelial Cells

TU Qian1; YE Yong1; YANG Dandan2

doi:10.13288/j.11-2166/r.2013.16.028

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Effect of Tanshinone Ⅱ_A on the Expression of Endothelial Nitric Oxide Synthase and Phosphoinositide 3-Kinase in Human Umbilical Vein Endothelial Cells

更新时间：2021-11-17

- Effect of Tanshinone Ⅱ_A on the Expression of Endothelial Nitric Oxide Synthase and Phosphoinositide 3-Kinase in Human Umbilical Vein Endothelial Cells
- Journal of Traditional Chinese Medicine Vol. 54, Issue 16, Pages: 1412-1414(2013)
- 作者机构：
  
  1. 江汉大学医学院
  2. 山东省乳山市人民医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2013.16.028
  CLC： R285.5
- Published：2013
- 稿件说明：
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[1]凃乾,叶勇,杨丹丹.丹参酮Ⅱ_A对人脐静脉内皮细胞系一氧化氮合酶和磷脂酰肌醇-3激酶的影响[J].中医杂志,2013,54(16):1412-1414.

TU Qian1, YE Yong1, YANG Dandan2. Effect of Tanshinone Ⅱ_A on the Expression of Endothelial Nitric Oxide Synthase and Phosphoinositide 3-Kinase in Human Umbilical Vein Endothelial Cells[J]. Journal of Traditional Chinese Medicine2013, 54(16): 1412-1414. DOI: 10.13288/j.11-2166/r.2013.16.028.
[1]凃乾,叶勇,杨丹丹.丹参酮Ⅱ_A对人脐静脉内皮细胞系一氧化氮合酶和磷脂酰肌醇-3激酶的影响[J].中医杂志,2013,54(16):1412-1414. DOI： 10.13288/j.11-2166/r.2013.16.028.

TU Qian1, YE Yong1, YANG Dandan2. Effect of Tanshinone Ⅱ_A on the Expression of Endothelial Nitric Oxide Synthase and Phosphoinositide 3-Kinase in Human Umbilical Vein Endothelial Cells[J]. Journal of Traditional Chinese Medicine2013, 54(16): 1412-1414. DOI: 10.13288/j.11-2166/r.2013.16.028. DOI：

摘要

目的探讨丹参酮ⅡA促血管生成的作用机制。方法将人脐静脉内皮细胞系（HUV-EC-C）分为空白组、二甲基亚枫（DMSO

终浓度1%）组、血管内皮生长因子（VEGF

终浓度5μg/L）组、丹参酮ⅡA（终浓度6mg/L）组

分别加入相应药物48h后

镜下观察各组细胞形态

并采用蛋白质印迹法测定各组细胞内皮型一氧化氮合酶（eNOS）、磷酯酰肌醇-3激酶（PI3K）表达情况。结果 VEGF组和丹参酮ⅡA组HUV-EC-C细胞形态饱满

丰度明显增加

细胞间隙缩小

均未见脱落。与空白组比较

VEGF组和丹参酮ⅡA组细胞eNOS、PI3K蛋白表达均显著增强（P<0.05或P<0.05）。结论丹参酮ⅡA能提高HUV-EC-C内PI3K和eNOS蛋白表达

可能是其促血管生成的作用机制之一。

Abstract

Objective To study the mechanism of Tanshinone ⅡA（TsⅡA） in promoting angiogenesis.Methods The cultured human umbilical vein endothelial cells（HUVEC-C） were randomized into the blank group

dimethyl sulfoxide（DMSO

a final concentration of 1%） group

vascular endothelial growth factor（VEGF

a final concentration of 5 μg/L） group and TsⅡA（a final concentration of 6 mg/L） group.The cell morphology was observed in each group 48h after the corresponding drugs were added.The expression of endothelial nitric oxide synthase（eNOS） and phosphoinositide 3-kinase（PI3K） was detected by Western blotting.Results The HUVEC-C cells in the VEGF group and TsⅡA group were fully shaped and significantly abundant with a narrow cell gap and no shedding.Comparing with the blank group

the expression of eNOS and PI3K in the VEGF group and TsⅡA group was significantly increased（P<0.05 or P<0.01）.Conclusion TsⅡA can increase the expression of eNOS and PI3K in HUVEC-C

which may be the mechanism of angiogenesis.

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Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University

Beijing University of Chinese Medicine

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Effect of Tanshinone ⅡA on the Expression of Endothelial Nitric Oxide Synthase and Phosphoinositide 3-Kinase in Human Umbilical Vein Endothelial Cells

DOI：10.13288/j.11-2166/r.2013.16.028

摘要

Abstract

关键词

Keywords

references

Effect of Tanshinone Ⅱ_A on the Expression of Endothelial Nitric Oxide Synthase and Phosphoinositide 3-Kinase in Human Umbilical Vein Endothelial Cells