Effect of Qubi Formula on the Immunomodulatory Factors in TypeⅡ Collagen-Induced Arthritis Rats

FU Xiaowei; ZHAO Hongyan; YU Zheng; LIU Meijie; WANG Ying; ZHOU Shengfang; SONG Changheng; JU Dahong

doi:10.13288/j.11-2166/r.2013.20.025

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Effect of Qubi Formula on the Immunomodulatory Factors in TypeⅡ Collagen-Induced Arthritis Rats

更新时间：2021-11-17

- Effect of Qubi Formula on the Immunomodulatory Factors in TypeⅡ Collagen-Induced Arthritis Rats
- Journal of Traditional Chinese Medicine Vol. 54, Issue 20, Pages: 1770-1773(2013)
- 作者机构：
  
  1. 陕西中医学院
  2. 中国中医科学院中医基础理论研究所
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2013.20.025
  CLC： R285.5
- Published：2013
- 稿件说明：
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[1]付小卫,赵宏艳,于峥,刘梅洁,王莹,周晟芳,宋长恒,鞠大宏.祛痹方对Ⅱ型胶原诱导的免疫性关节炎大鼠免疫调节因子的影响[J].中医杂志,2013,54(20):1770-1773.

FU Xiaowei, ZHAO Hongyan, YU Zheng, et al. Effect of Qubi Formula on the Immunomodulatory Factors in TypeⅡ Collagen-Induced Arthritis Rats[J]. Journal of Traditional Chinese Medicine2013, 54(20): 1770-1773.
[1]付小卫,赵宏艳,于峥,刘梅洁,王莹,周晟芳,宋长恒,鞠大宏.祛痹方对Ⅱ型胶原诱导的免疫性关节炎大鼠免疫调节因子的影响[J].中医杂志,2013,54(20):1770-1773. DOI： 10.13288/j.11-2166/r.2013.20.025.

FU Xiaowei, ZHAO Hongyan, YU Zheng, et al. Effect of Qubi Formula on the Immunomodulatory Factors in TypeⅡ Collagen-Induced Arthritis Rats[J]. Journal of Traditional Chinese Medicine2013, 54(20): 1770-1773. DOI： 10.13288/j.11-2166/r.2013.20.025.

摘要

目的探讨祛痹方对Ⅱ型胶原诱导的免疫性关节炎（CIA）大鼠的作用及免疫调节机制。方法 SD大鼠72只随机分为空白对照组12只

模型组、雷公藤多苷组、祛痹方高剂量组、祛痹方低剂量组各15只。除空白对照组外其余各组采用尾根部皮下注射Ⅱ型胶原与不完全弗氏佐剂的方法诱导出CIA模型

造模第15天祛痹方高、低剂量组分别给予祛痹方混悬液6.66g/（kg·d）、3.33g/（kg·d）

雷公藤多苷组给予雷公藤多苷混悬液9.68mg/（kg·d）

空白对照组和模型组大鼠等体积纯净水

各组每天灌胃1次

连续4周。观察各组大鼠关节病理损伤并进行半定量评分

检测血浆中抗Ⅱ型胶原抗体（COL-2）、白细胞介素-1β（IL-1β）、白细胞介素6（IL-6）含量。结果模型组大鼠膝关节病理积分

血浆COL-2、IL-1β、IL-6含量较空白对照组明显升高（P<0.05或P<0.01）;与模型组比较

祛痹方高剂量组与雷公藤多苷组大鼠关节病理积分

血浆COL-2、IL-1β、IL-6含量明显降低（P<0.05或P<0.01）;祛痹方高剂量组大鼠关节病理积分

血浆COL-2、IL-1β水平亦明显低于雷公藤多苷组和祛痹方低剂量组（P<0.05或P<0.01）。结论高剂量祛痹方显著减轻了CIA大鼠膝关节损伤

其作用机理可能与降低血浆COL-2水平

下调血浆IL-1β、IL-6含量有关。

Abstract

Objective To study the therapeutic effect and Immunomodulatory mechanism of Qubi Formula（ImpedimentDispelling Formula）for typeⅡcollagen-induced arthritis（CIA）in rats.Methods Totally 72SD rats were randomized into the blank control group

model group

tripterygium glycosides group

Qubi Formula high-dose group and Qubi Formula lowdose group

with 12

15and 15in each.The CIA models were induced by subcutaneous injection of collagen typeⅡ and incomplete Freund’s adjuvant on the tail root of rats in all groups except the blank control group.On the 15th day of modeling

the Qubi Formula high-dose and low-dose groups were gavaged with 6.66g/（kg·d）and 3.33g/（kg·d）Qubi Formula suspension

the tripterygium glycosides group was gavaged with 9.68mg/（kg·d）tripterygium glycosides suspension and the blank control group and model group were gavaged with equal volume of pure water once daily for 4 weeks.The pathological damage of joints in each group was observed and semi-quantitative score was calculated.The contents of anti-type II collagen antibodies（COL-2）

interleukin-1β（IL-1β）and interleukin-6（IL-6）were detected.Results Comparing with the blank control group

the score of knee joint pathology and contents of COL-2

IL-1βand IL-6were significantly increased in the model group（P<0.05or P<0.01）.Comparing with the model group

the score of knee joint pathology and contents of COL2

IL-1βand IL-6were significantly decreased in the Qubi Formula high-dose group and tripterygium glycosides group（P< 0.05or P<0.01）.Comparing with the Qubi Formula low-dose group and tripterygium glycosides group

the score of knee joint pathology and contents of COL-2and IL-1βwere significantly decreased in the Qubi Formula high-dose group（P<0.05or P<0.01）.Conclusion High-dose Qubi Formula can obviously alleviate knee injury in CIA rats that may be relevant to reducing the COL-2level and down-regulating the contents of IL-1βand IL-6.

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Related Institution

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Affiliated Hospital of Jiangxi University of Chinese Medicine

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