Objective To research the possible molecular mechanism of Xiaoyao Pill（Free Wanderer Pill）containing serum on the endocrine function of ovarian granulose cells.Methods Twelve Wistar rats were randomized into Xiaoyao Pill lowdose

middle-dose and high-dose groups（1ml/100g

2ml/100g and 3ml/100g Xiaoyao Pill suspension was gavaged to each rat daily）and control group（each rat was gavaged with 2ml/100g normal saline daily）.The administration lasted for 4days.Xiaoyao Pill low-dose

middle-dose and high-dose rat containing serum and normal rat serum was prepared by collecting blood from the femoral artery 1 hafter the last administration.The follicular fluid of in vitro fertilization and embryo transfer（IVFET）patients was collected to extract granule cells and culture primary cells for 24 hours.Xiaoyao Pill low-dose

middle-dose and high-dose rat containing serum and normal rat serum was incubated with cultured human ovarian granulosa cells together for 48 hours respectively and divided into Xiaoyao Pill low-dose

middle-dose and high-dose groups and control group.The levels of estradiol（E2）

progesterone（P） and cyclic adenosine monophosphate（cAMP）in culture solution were detected with radioimmunoassay.The Western blot and Real-time PCR were used to detect the expressions of follicle stimulating hormone receptor（FSHR）protein

FSHR mRNA

P450 aromatase mRNA and peroxisome proliferator-activated receptor-γ（PPARγ）mRNA in ovarian granulosa cells.Results Comparing with the control group

the levels of E2 and cAMP in Xiaoyao Pill middle-dose and high-dose groups were significantly increased

the FSHR protein expression in Xiaoyao Pill low-dose

middledose and high-dose groups was significantly increased

the expressions of FSHR mRNA and P450 aromatase mRNA in Xiaoyao Pill high-dose group were significantly increased and the PPARγ mRNA expression in Xiaoyao Pill middle-dose group was significantly decreased（P<0.05or P<0.01）.Comparing with Xiaoyao Pill low-dose group

the levels of E2

cAMP

FSHR protein and FSHR mRNA were significantly increased in Xiaoyao Pill high-dose group（P<0.01）.Conclusion Xiaoyao Pill can make FSH promote follicular development and ovulation by increasing the expressions of FSHR protein and FSHR mRNA in ovarian granulosa cells and promote the endocrine function of ovarian granulosa cells by regulating the levels of FSHR

cAMP

P450 aromatase and E2.