Effect of Feibitong Formula on the Collagen and Related Factors in Rats with Pulmonary Fibrosis

FAN Maorong; LI Yanyan; ZHANG Qiong; WANG Shuchen; ZHANG Yanping; ZHANG Xuli

doi:10.13288/j.11-2166/r.2013.21.025

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Effect of Feibitong Formula on the Collagen and Related Factors in Rats with Pulmonary Fibrosis

更新时间：2021-11-17

- Effect of Feibitong Formula on the Collagen and Related Factors in Rats with Pulmonary Fibrosis
- Journal of Traditional Chinese Medicine Vol. 54, Issue 21, Pages: 1859-1862(2013)
- 作者机构：
  
  1. 中国中医科学院西苑医院
  2. 中国科学院半导体研究所
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2013.21.025
  CLC： R285.5
- Published：2013
- 稿件说明：
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[1]樊茂蓉,李艳艳,张琼,王书臣,张燕萍,张旭丽.肺痹通方对肺纤维化大鼠胶原蛋白及相关因子的影响[J].中医杂志,2013,54(21):1859-1862.

FAN Maorong, LI Yanyan, ZHANG Qiong, et al. Effect of Feibitong Formula on the Collagen and Related Factors in Rats with Pulmonary Fibrosis[J]. Journal of Traditional Chinese Medicine2013, 54(21): 1859-1862.
[1]樊茂蓉,李艳艳,张琼,王书臣,张燕萍,张旭丽.肺痹通方对肺纤维化大鼠胶原蛋白及相关因子的影响[J].中医杂志,2013,54(21):1859-1862. DOI： 10.13288/j.11-2166/r.2013.21.025.

FAN Maorong, LI Yanyan, ZHANG Qiong, et al. Effect of Feibitong Formula on the Collagen and Related Factors in Rats with Pulmonary Fibrosis[J]. Journal of Traditional Chinese Medicine2013, 54(21): 1859-1862. DOI： 10.13288/j.11-2166/r.2013.21.025.

摘要

目的探讨肺痹通方治疗肺纤维化的作用机制。方法将60只SD大鼠随机分为正常对照组、模型组、激素组、肺痹通低剂量组及肺痹通高剂量组各12只

除正常对照组外其余各组大鼠气管内注射博来霉素建立肺纤维化大鼠模型

造模后28天激素组给予强的松片3mg/（kg·d）灌胃

肺痹通低、高剂量组分别给予肺痹通方10g/（kg·d）、20g/（kg·d）灌胃

正常对照组和模型组给予2ml/d蒸馏水灌胃

连续21天。观察各组大鼠肺组织病理形态学

检测血清层粘连蛋白（LN）、转化生长因子-β1（TGF-β1）、谷胱甘肽（GSH）及肺组织Ⅰ型胶原、Ⅲ型胶原、基质金属蛋白酶（MMP-1）和其组织抑制因子（TIMP-1）水平的变化。结果与正常对照组比较

模型组大鼠血清LN、TGF-β1及肺组织MMP-1、TIMP-1、Ⅰ型胶原、Ⅲ型胶原水平升高（P<0.05）。激素组与肺痹通高剂量组LN水平低于模型组（P<0.05）;各组大鼠血清GSH水平比较差异均无统计学意义（P>0.05）。激素组及肺痹通高、低剂量组MMP-1、TIMP-1、I型胶原水平均较模型组显著降低（P<0.05）;肺痹通高剂量组MMP-1水平较肺痹通低剂量组明显升高（P<0.05）;激素组较肺痹通低剂量组TIMP-1水平明显降低（P<0.05）。结论肺痹通方可能通过调节MMPs/TIMPs比例失衡

减少LN以及Ⅰ、Ⅲ型胶原纤维的表达

从而延缓肺纤维化进程。

Abstract

Objective To research the mechanism of Feibitong Formula （formula to treat pulmonary fibrosis）for pulmonary fibrosis.Methods Sixty rats were randomized into normal control group

model group

hormone group

Feibitong low-dose group and Feibitong high-dose group

with 12 in each.The pulmonary fibrosis models were established by intratracheal injection of bleomycin in all groups but normal control group.The hormone group

Feibitong low-dose group

Feibitong highdose group was gavaged with 3mg/kg Prednisone Tablets

10g/kg Feibitong Formula

20g/kg Feibitong Formula respectively

normal control group and model group was given 2ml distilled water 28 days after modeling.The intragastric administration lasted for 21 days.The pathological form in lung tissue was observed.The levels of laminin（LN）

transforming growth factor-β1（TGF-β1）

glutathione（GSH）

typeⅠ and typeⅢ collagens in lung tissues

matrix metalloproteinase-1（MMP-1） and tissue inhibitor of metalloproteinase-1（TIMP-1） were detected.Results Comparing with the normal control group

the levels of LN

TGF-β1

MMP-1

TIMP-1

Ⅰ and Ⅲ type collagens in lung tissues in the model group were significantly increased（P<0.05）.Comparing with the model group

the LN levels were significantly decreased in the hormone group and Feibitong high-dose group（P<0.05）.There was no significant difference between groups in the GSH level（P>0.05）.Comparing with the model group

the levels of MMP-1

TIMP-1 and typeⅠcollagen were significantly decreased in the hormone group and Feibitong high-dose and low-dose groups（P<0.05）.The MMP-1 level in the Feibitong high-dose group was significantly higher than that in the Feibitong low-dose group（P<0.05）.The TIMP-1level in the hormone group was significantly lower than that in the Feibitong low-dose group（P<0.05）.Conclusion Feibitong Formula can delay the process of pulmonary fibrosis by regulating the imbalance of MMPs/TIMPs and reducing the expressions of LN and typeⅠand typeⅢcollagens.

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Related Institution

The First Hospital of Hunan University of Chinese Medicine

College of Acupuncture-Moxibustion-Tuina and Rehabilitation，Hunan University of Chinese Medicine

School of Acupuncture，Moxibustion and Tuina， Fujian University of Traditional Chinese Medicine

The Second Affiliated Hospital of Anhui University of Chinese Medicine

The Second Clinical Medical College of Anhui University of Chinese Medicine

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