Objective To study the effect of concentrated autologous bone marrow aspirate transplantation（ CABMAT） combined with kidney-supplementing blood-quickening herbs on fracture healing in osteoporotic fractures（ OPF）. Methods Totally 100 white rabbits were randomized into the sham operation group

model group

transplantation group

Chinese medicinal group and combination group

with 20 in each. The osteoporotic models were established by ovariectomy for three months in all groups except the sham operation group. Then the fracture models were established by amputating 3mm radial bones of left forelegs in all groups. The concentrated autologous bone marrow（ 2ml / rabbit） was percutaneously injected into the fracture fragments in the transplantation group. The Chinese medicinal group was gavaged with 10ml /（ kg·d） kidney-supplementing blood-quickening herbs. The combination group was given bone marrow transplantation and intragastric administration of kidney-supplementing blood-quickening herbs. The sham operation group and model group were gavaged with the same amount of normal saline for 8 weeks. The general observation

X-ray and CT scanning were conducted in the bone tissue of fracture in the 2nd

4th

6th and 8th week of treatment. The CT values of bone callus were measured and the morphological changes were observed with HE staining. Results The combination group

transplantation group and Chinese medicinal group were superior to the model group in the general observation

X-ray and HE staining

especially the combination group. Comparing with the sham operation group

the CT values of bone callus were significantly decreased in the model group in the 2nd

4th

6th and 8th week of treatment（ P < 0. 01）. Comparing with the Chinese medicinal group

the CT values of bone callus were significantly increased in the transplantation group and combination group in the 2nd

4th

6th and 8th week of treatment except in the transplantation group in the 8th week of treatment（ P < 0. 01）. Comparing with the transplantation group

the CT values of bone callus were significantly increased in the combination group in the 4th

6th and 8th week of treatment（ P < 0. 01）. Conclusion The combo therapy of CABMAT and kidneysupplementing blood-quickening herbs can promote and accelerate the fracture healing in OPF.