The Activity Changes of 8-Iso-PGF2α in AFLD Rats and Timeliness of Yigan Jiangzhi Formula

ZHAO Yuanhong; YANG Caixia; LI Quanzheng; LI Zheng; DONG Chengchao; LI Xiaojiang; YIN Jihong; HOU Shanshan; ZHANG Xiaorui; JIA Yingjie

doi:10.13288/j.11-2166/r.2014.20.016

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The Activity Changes of 8-Iso-PGF2α in AFLD Rats and Timeliness of Yigan Jiangzhi Formula

更新时间：2021-11-17

- The Activity Changes of 8-Iso-PGF2α in AFLD Rats and Timeliness of Yigan Jiangzhi Formula
- Journal of Traditional Chinese Medicine Vol. 55, Issue 20, Pages: 1768-1772(2014)
- 作者机构：
  
  1. 天津中医药大学第一附属医院
  2. 天津中医药大学研究生院
  3. 天津中医药大学针灸研究所
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2014.20.016
  CLC： R285.5
- Published：2014
- 稿件说明：
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[1]赵远红,杨彩霞,李全征,李正,董承超,李小江,尹纪红,侯珊珊,张小瑞,贾英杰.酒精诱导大鼠脂肪肝8-异前列腺素F2α活性变化及益肝降脂方的干预时效[J].中医杂志,2014,55(20):1768-1772.

ZHAO Yuanhong, YANG Caixia, LI Quanzheng, et al. The Activity Changes of 8-Iso-PGF2α in AFLD Rats and Timeliness of Yigan Jiangzhi Formula[J]. Journal of Traditional Chinese Medicine2014, 55(20): 1768-1772.
[1]赵远红,杨彩霞,李全征,李正,董承超,李小江,尹纪红,侯珊珊,张小瑞,贾英杰.酒精诱导大鼠脂肪肝8-异前列腺素F2α活性变化及益肝降脂方的干预时效[J].中医杂志,2014,55(20):1768-1772. DOI： 10.13288/j.11-2166/r.2014.20.016.

ZHAO Yuanhong, YANG Caixia, LI Quanzheng, et al. The Activity Changes of 8-Iso-PGF2α in AFLD Rats and Timeliness of Yigan Jiangzhi Formula[J]. Journal of Traditional Chinese Medicine2014, 55(20): 1768-1772. DOI： 10.13288/j.11-2166/r.2014.20.016.

摘要

目的观察8-异前列腺素F2α（8-iso-PGF2α）在大鼠酒精性脂肪肝病（AFLD）造模过程中的活性变化及与益肝降脂方干预的时效关系。方法将97只SD大鼠随机分为模型组22只和中药干预组75只

予高脂联合梯度酒精灌胃建立AFLD模型;中药干预组随机分为第3周组、第6周组、第9周组

分别于造模第3周、第6周、第9周起给予益肝降脂方浸膏1ml（含生药1.33g/ml）灌胃

模型组给予等剂量生理盐水

至造模12周。各组在不同时间点检测血清8-iso-PGF2α活性

丙氨酸氨基转移酶（ALT）、门冬氨酸氨基转移酶（AST）、甘油三酯（TG）、总胆固醇（TC）含量

肝组织丙二醛（MDA）、黄嘌呤氧化酶（XOD）活性;观察肝组织病理变化。结果随着造模时间延长

大鼠血清8-iso-PGF2α活性与ALT、AST、TG、TC水平明显升高（P

0.05或P

0.01）;第3周组（3

周）和（3

2周）与第9周组（9

2周）比较

8-iso-PGF2α、ALT、AST、TC、MDA、XOD水平明显降低（P

0.05或P

0.01）。光镜下模型组脂肪变性随着造模时间延长呈现逐渐加重趋势

至12周末呈中、重度脂肪变性伴水样变性3

度

各中药干预组病变程度均较模型组减轻

第3周组较第9周组（9

2周）脂肪变性及水样变性程度减轻。结论大鼠血清8-iso-PGF2α活性、肝损伤程度与造模时间正相关;益肝降脂方对AFLD肝损伤具有保护作用

且干预时间越早效果越明显

其机制可能与降低8-iso-PGF2α活性

减轻氧化应激及脂质过氧化相关。

Abstract

Objective To observe the activity changes of 8-iso-prostaglandin F2α（ 8-iso-PGF2α） in rats with alcoholic fatty liver disease（ AFLD） and time-effect relationship with Yigan Jiangzhi Formula（ liver-supplementing lipid-lowering formula）. Methods Totally 97 cases of SD rats were randomized into the model group of 22 cases and Chinese medicine group of 75 cases. The AFLD rat models were established with high-fat diet and graded ethanol feeding. The Chinese medicine group was divided into the 3rd week group

6th week group and 9th week group. The rats in the 3rd week group

6th week group and 9th week group were gavaged with 1ml extract of Yigan Jiangzhi Formula（ containing crude drug 1. 33 g /ml） from the 3rd week

6th week and 9th week respectively. The model group was given the same volume of normal saline. The rats were sacrificed in all groups at different time points. The activity of 8-iso-PGF2α and contents of alanine aminotransferase（ ALT）

aspartate aminotransferase（ AST）

triglyceride（ TG） and total cholesterol（ TC） in serum and the activities of malondialdehyde（ MDA） and xanthine oxidase（ XOD） in liver tissue were detected. The pathological changes in liver tissue were observed. Results The activity of 8-iso-PGF2α and the levels of ALT

AST

TG and TC in serum were significantly increased with the modeling time（ P <0.05 or P <0. 01）. Comparing with the 9th week group

the activity of 8-iso-PGF2α and the levels of ALT

AST

MDA and XOD in the 3rd week group（ 3rd to 6th week and 3rd to 12 th week） were significantly decreased（ P <0. 05 or P <0.01）. Under light microscope

the steatosis was gradually increased with the modeling time in the model group. Moderate to severe steatosis accompanied by 3-degree to 4-degree hydropic degeneration could be seen in the model group at the end of the 12 th week. Comparing with the model group

the pathological changes were relieved in all Chinese medicine groups. Comparing with the 3rd week group

the steatosis and hydropic degeneration was significantly decreased in the 9th week group. Conclusion The activity of 8-iso-PGF2α and the degree of liver damage are positively correlated with modeling time. Yigan Jiangzhi Formula has a protective effect on AFLD. The earlier the intervention is given

the better effect is gained. Its mechanism may be relevant to reducing the activity of8-iso-PGF2α

oxidative stress and lipid peroxidation.

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