Objective To explore the effect of Jinxin Oral Liquid containing serum on signal transduction pathway of respiratory syncytial virus（ RSV） and its possible action mechanism. Methods Sprague-Dawley rats were gavaged 76. 8 g /（ kg·d） of Jinxin Oral Liquid or 128. 1 mg /（ kg·d） of ribavirin granules for 3 days to prepare the Jinxin Oral Liquid containing serum and ribavirin containing serum

respectively. RAW264. 7 cells were infected by RSV and then were divided into the model group

ribavirin group

and Jinxin Oral Liquid group. There is also a normal control group with normal cells. The Jinxin Oral Liquid group and ribavirin group were intervened by 2. 5 ml of Jinxin Oral Liquid containing serum or ribavirin respectively

while the normal control group and the model group were given 2. 5 ml of 2% FBS. The cells were collected

and the expressions of TLR3

TANK-binding kinase1（ TBKl）and interferon regulatory factor3（ IRF3） mRNA of RAW264. 7 cells were detected 12 hours later. Results Twelve hours later after RAW264. 7 cells were infected by RSV

TLR3

TBK1 and IRF3 mRNA were induced and high protein expression was showed（ P < 0. 01）. Jinxin Oral Liquid containing serum and ribavirin could down-regulate the expressions of TLR3

TBK1 and IRF3 mRNA and their proteins（ P < 0. 01）. Jinxin Oral Liquid was superior to ribavirin in down-regulating the expression of TLR3 protein（ P < 0. 05）. Conclusion RSV can cause the activation of TLR3 signal transduction pathway and up-regulate the expressions of TBK1 and IRF3. Jinxin Oral Liquid Containing Serum can inhibit TLR3 signal transduction pathway to resist RSV.