Effect of Xingnaojing on Rat Nerve Cell Autophagy after Cerebral Ischemia Reperfusion

HUANG Guihua; YU Jiufeng; MENG Honghua; HUANG Liqiong; ZHOU Heng; LIU Li; LIN Huash-eng; SHEN Wenyi

doi:10.13288/j.11-2166/r.2014.22.017

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Effect of Xingnaojing on Rat Nerve Cell Autophagy after Cerebral Ischemia Reperfusion

更新时间：2021-11-17

- Effect of Xingnaojing on Rat Nerve Cell Autophagy after Cerebral Ischemia Reperfusion
- Journal of Traditional Chinese Medicine Vol. 55, Issue 22, Pages: 1952-1956(2014)
- 作者机构：
  
  1. 广西中医药大学第一附属医院
  2. 江苏省涟水县人民医院
  3. 广西中医药大学研究生学院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2014.22.017
  CLC： R285.5
- Published：2014
- 稿件说明：
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[1]黄贵华,余九峰,蒙红华,黄丽琼,周衡,刘莉,林华胜,沈文沂.醒脑静对脑缺血再灌注大鼠神经细胞自噬作用的影响[J].中医杂志,2014,55(22):1952-1956.

HUANG Guihua, YU Jiufeng, MENG Honghua, et al. Effect of Xingnaojing on Rat Nerve Cell Autophagy after Cerebral Ischemia Reperfusion[J]. Journal of Traditional Chinese Medicine2014, 55(22): 1952-1956.
[1]黄贵华,余九峰,蒙红华,黄丽琼,周衡,刘莉,林华胜,沈文沂.醒脑静对脑缺血再灌注大鼠神经细胞自噬作用的影响[J].中医杂志,2014,55(22):1952-1956. DOI： 10.13288/j.11-2166/r.2014.22.017.

HUANG Guihua, YU Jiufeng, MENG Honghua, et al. Effect of Xingnaojing on Rat Nerve Cell Autophagy after Cerebral Ischemia Reperfusion[J]. Journal of Traditional Chinese Medicine2014, 55(22): 1952-1956. DOI： 10.13288/j.11-2166/r.2014.22.017.

摘要

目的探讨醒脑静对脑缺血再灌注（CIR）过程中神经细胞自噬的疗效及可能作用机制。方法用栓线法制备90只大脑中动脉栓塞大鼠模型

选取符合要求的模型大鼠随机分为2大组:MRI组（20只）和WB组（70只）

MRI组随机分为2小组:模型对照组10只

醒脑静组10只;WB组随机分为7组:模型组

溶媒组

醒脑静组

3-MA组

3-MA+醒脑静组

雷帕霉素（Rapa）组

Rapa+醒脑静组（每组10只）。用磁共振成像检测MRI组大鼠用药前后模型脑梗的体积

计算脑梗体积占全脑体积的比例;用免疫印迹法检测WB组大鼠细胞内自噬相关蛋白的表达。结果 MRI组:各组大鼠给药24h后的脑梗体积比例均大于给药前的脑梗体积比例

差异具有统计学意义（P<0.05）;醒脑静组的脑梗体积比例小于模型对照组

差异具有统计学意义（P<0.05）。WB组:与模型组相比

醒脑静组、3-MA组与3-MA+醒脑静组的Beclin1和LC3的表达量均降低

Bcl-2的表达量升高

差异具有统计学意义（P<0.05）

且3-MA组+醒脑静组优于3-MA组与醒脑静组（P<0.05）;Rapa组与Rapa组+醒脑静组Beclin1和LC3的表达量均高于模型组、醒脑静组、3-MA组与3-MA组+醒脑静组

Bcl-2表达量低于模型组、醒脑静组、3-MA+醒脑静组（P<0.05）

且Rapa+醒脑静组Beclin1和LC3低于Rapa组

Bcl-2高于Rapa组（P<0.05）。Bcl-2与Beclin1之间的表达量呈负相关。结论醒脑静可抑制大鼠脑缺血再灌注过程中的自噬反应

提高受损神经细胞的存活率。

Abstract

Objective To explore the therapeutic effect and possible mechanism of Xingnaojing on nerve cell autophagy after cerebral ischemia reperfusion（ CIR）. Methods Rat models of middle cerebral artery（ MCA） ischemia reperfusion were prepared with line embolism. Totally 90 2-score models were chosen by Longa Score method and were randomized into the magnetic resonance imaging（ MRI） group（ 20 mice） and Western blot（ WB） group（ 70mice）. Then the rats in MRI group were randomized into the model control group and Xingnaojing group

with 10 in each. The rats in WB group were randomized into the model group

solvent group

Xingnaojing group

3-MA group

3-MA and Xingnaojing group

Rapa group

and Rapa and Xingnaojing group

with 10 in each. The infarct volume of rats in MRI group was measured with MRI before and after medication

and the infarct volume ratio was calculated.The expression of autophagy related proteins of rats in WB group was detected with immunoblotting. Results The infarct volume ratio in MRI group was significantly increased 24 hours after medication（ P < 0. 05）. The infarct volume ratio in Xingnaojing group was significantly smaller than that in the model control group（ P < 0. 05）. In WB group

comparing with the model group

the expressions of Beclin1 and LC3 were significantly decreased but the expression of Bcl-2 was significantly increased in Xingnaojing group

3-MA group

and 3-MA and Xingnaojing group（ P <0.05）

especially in 3-MA and Xingnaojing group（ P <0.05）. The expressions of Beclin1 and LC3 in Rapa group and Rapa and Xingnaojing group were significantly higher than those in the model group

Xingnaojing group

3-MA group

and 3-MA and Xingnaojing group（ P < 0. 05）. The expression of Bcl-2 in Rapa group and Rapa and Xingnaojing group was significantly lower than that in the model group

3-MA group

and 3-MA and Xingnaojing group（ P < 0. 05）. The expressions of Beclin1 and LC3 in Rapa and Xingnaojing group was significantly lower than those in Rapa group（ P < 0. 05）. The expression of Bcl-2 in Rapa and Xingnaojing group was significantly higher than that in Rapa group（ P < 0. 05）. The expression of Bcl-2 and Beclin1 was negatively correlated. Conclusion Xingnaojing can inhibit rat autophagy during cerebral ischemia and improve survival rate of injured nerve cells.

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Related Institution

Graduate School of Tianjin Medical University

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Tianjin Academy of Traditional Chinese Medicine

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