Objective To observe influence of Fufang Zhebei Granules（ FFZBG） on proliferation and apoptosis of human colorectal cancer HCT-116 cells and discuss the possible mechanism. Methods A total of 40 Wistar rats were randomly assigned to control group and high

middle

low-dose FFZBG group

with 10 in each. For 7 days

high

middle

low-dose FFZBG group were given 8

4

2 g /（ kg · d） drug solution by gavage

while the control group was given 0. 1 ml /10 g normal saline. One hour after the last gavage

drug-containing serum was prepared. MTT method was applied to detect cell inhibitory rate of HCT-116 cells intervened by drug-containing serum of different dosage. The apoptosis rates of HCT-116 cells were detected quatitatively by flow cytometry. Western blot was used to examine the influence on expression of MDM2

p53

p21 proteins. Results The cell inhibitory rates of high

middle

low-dose FFZBG group were 40. 00%

42. 37% and 26. 78% respectively

while apoptosis rates were18. 75%

20. 00% and 15. 32% respectively

which were higher than that of the control group（ P < 0. 05）. At 48 h

the expression of MDM2

p53

p21 protein in high-dose FFZBG group

MDM2 protein in middle-dose FFZBG group and MDM2

p53 protein in low-dose FFZBG group were higher than that of the control group（ P < 0. 01）. At 96 h

the expression of MDM2 protein of high

middle

low-dose FFZBG group was significantly lower than that at 48 h

while the expression of p53 protein was higher（ P < 0. 01）. Conclusion Fufang Zhebei Granules can inhibit the proliferation of HCT-116 cells and promote apoptosis

with the possible mechanism of up-regulating the MDM2-p53 signal pathway.